Department of Bio and Brain Engineering, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Lab Chip. 2011 Feb 7;11(3):413-8. doi: 10.1039/c0lc00148a. Epub 2010 Nov 12.
A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells s(-1) and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.
一种温和但快速的低压力、高通量血小板纯化方法具有重要的临床和生物技术应用价值。然而，目前用于分选血小板的方法需要外部物理场、专用缓冲液或高剪切应力等苛刻的分离条件，这些条件往往会导致血小板受到刺激。在这里，我们报告了在更宽的通道中使用电泳尺寸分离及其并行化来提高其高通量能力，同时保持生理剪切应力范围。我们展示了一种由 10 个堆叠的宽通道电泳装置组成的并行设备，其吞吐量为 290 万个细胞/s，血小板纯度为 76.8%。电泳尺寸分离在宽通道中的应用也便于通过分离血栓和斑块来实现无堵塞的分离。宽通道电泳具有温和、快速、无堵塞的分选稀有血细胞的潜力，具有极高的吞吐量能力。
