Vanderbilt University, Department of Chemistry, Nashville, TN, USA.
IET Syst Biol. 2010 Nov;4(6):416-27. doi: 10.1049/iet-syb.2010.0012.
The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental set-up and control parameters and online desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signalling pathways.
微流控细胞捕获装置与离子淌度-质谱联用，有望实时阐明小群细胞对旁分泌信号、代谢物水平变化以及药物和毒素传递的动态反应。初步的甲醇中肽的实验研究和记录酵母与 Jurkat 细胞与共孵育液相互作用的实验，确定了仪器设置和控制参数以及在线脱盐程序。大量初步实验验证了这一新型仪器平台。本文还讨论了未来展望和潜在应用，具体说明了该仪器如何用于细胞代谢和信号通路的显著相互依存的动态内部工作的全自动系统生物学研究。
