Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Biochemistry (Mosc). 2010 Aug;75(8):1052-4. doi: 10.1134/s0006297910080158.
A mutation in the vma2 gene disturbing V-ATPase function in the yeast Saccharomyces cerevisiae results in a five- and threefold decrease in inorganic polyphosphate content in the stationary and active phases of growth on glucose, respectively. The average polyphosphate chain length in the mutant cells is decreased. The mutation does not prevent polyphosphate utilization during cultivation in a phosphate-deficient medium and recovery of its level on reinoculation in complete medium after phosphate deficiency. The content of short chain acid-soluble polyphosphates is recovered first. It is supposed that these polyphosphates are less dependent on the electrochemical gradient on the vacuolar membrane.
在酵母酿酒酵母中，vma2 基因突变干扰 V-ATPase 的功能，导致在葡萄糖生长的静止期和生长期分别减少无机多磷酸盐含量的五倍和三倍。突变细胞中的多聚磷酸盐链长度降低。该突变不阻止在磷酸盐缺乏的培养基中培养期间多磷酸盐的利用，并且在磷酸盐缺乏后再接种到完全培养基中恢复其水平。首先恢复短链酸溶性多磷酸盐的含量。据推测，这些多磷酸盐对液泡膜上的电化学梯度的依赖性较小。
