Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.
J Oral Pathol Med. 2011 Feb;40(2):135-42. doi: 10.1111/j.1600-0714.2010.00967.x. Epub 2010 Nov 15.
The presence of cancer stem cell (CSC) antigens can be evidenced in some human tumors by phenotypic analysis through immunostaining. This study aims to identify a putative CSC immunophenotype in oral squamous cell carcinoma (OSCC) and determine its influence on prognosis.
The following data were retrieved from 157 patents: age, gender, primary anatomic site, smoking and alcohol intake, recurrence, metastases, histologic classification, treatment, disease-free survival (DFS), and overall survival (OS). An immunohistochemical study for CD44 and CD24 was performed in a tissue microarray of 157 paraffin blocks of OSCCs.
In univariate analysis, the immunostaining pattern showed significant influences in relation to OS for alcohol intake and treatment, as well as for the CD44(+) and CD44(-) /CD24(-) immunophenotypes. The multivariate test confirmed these associations.
Based on our results, the CD44 immunostaining and the absence of immunoexpression of these two investigated markers can be used in combination with other clinicopathologic information to improve the assessment of prognosis in OSCC.
通过免疫染色的表型分析，可以在一些人类肿瘤中证实癌症干细胞 (CSC) 抗原的存在。本研究旨在确定口腔鳞状细胞癌 (OSCC) 中可能存在的 CSC 免疫表型，并确定其对预后的影响。
从 157 名患者中检索以下数据：年龄、性别、原发解剖部位、吸烟和饮酒史、复发、转移、组织学分类、治疗、无病生存 (DFS) 和总生存 (OS)。对 157 例 OSCC 石蜡块的组织微阵列进行了 CD44 和 CD24 的免疫组织化学研究。
在单因素分析中，免疫染色模式与 OS 相关的酒精摄入和治疗以及 CD44(+)和 CD44(-)/CD24(-)免疫表型有显著影响。多因素检验证实了这些关联。
根据我们的结果，CD44 免疫染色以及这两个研究标记物的免疫表达缺失可以与其他临床病理信息结合使用，以改善 OSCC 预后评估。
