Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
Biochem Biophys Res Commun. 2011 Apr 1;407(1):28-33. doi: 10.1016/j.bbrc.2011.02.084. Epub 2011 Feb 20.
Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133(+) cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions.
口腔鳞状细胞癌 (OSCC) 对常规化疗或放疗的耐药性可能归因于癌症干细胞 (CSC)。新型抗癌药物的开发需要一种简单的方法来富集 CSC。CSC 可以从 OSCC 细胞系中富集，例如，在无血清细胞培养基 (SFM) 中培养后。在我们的研究中，我们分析了四种 OSCC 细胞系中 CSC 的存在情况。CSC 样细胞不能用 SFM 富集。然而，从全克隆集落获得的细胞系表现出 CSC 样特性，例如细胞增殖率降低，与亲本谱系的细胞相比，对紫杉醇的敏感性降低。此外，这些细胞系差异表达 CSC 标志物 CD133，其在 OSCC 组织中也上调。有趣的是，OSCC 组织中的 CD133(+)细胞表达很少或没有 Ki67，Ki67 是细胞增殖标志物，也表明药物敏感性降低。我们的研究展示了一种从 OSCC 细胞系中分离 CSC 样细胞系的方法。这些 CSC 样细胞系可能成为体外条件下开发抗癌药物的新靶点。
