Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health Science, University of Copenhagen, Frederik V's vej 11, DK-2100, Copenhagen, Denmark.
J Anal Toxicol. 2010 Nov;34(9):549-54. doi: 10.1093/jat/34.9.549.
A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-(13)C(3) as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (∼25 to 8300 μM) and D-β-hydroxybutyrate levels (∼30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.
介绍了一种用于测定血液中酮体（β-羟丁酸、丙酮和乙酰乙酸）的气相色谱-质谱法（GC-MS）。该方法基于 D-β-羟丁酸的酶促氧化为乙酰乙酸，然后脱羧为丙酮，使用顶空 GC-MS 并用丙酮-（13）C（3）作为内标进行定量。所开发的方法对于丙酮+乙酰乙酸水平（约 25 至 8300 μM）和 D-β-羟丁酸水平（约 30 至 16500 μM）的日内和总日内相对标准偏差 < 10%。回收率从 98%到 107%不等，证明该方法适用于测量广泛浓度范围内的酮体。该方法已应用于疑似糖尿病或慢性酒精中毒患者酸中毒导致死亡的病例，以及已确定其他死因的病例。
Zotero 插件