Determination of ketone bodies in postmortem blood by head-space gas chromatography.
作者信息
Felby S, Nielsen E
机构信息
Institute of Forsenic Chemistry, University of Copenhagen, Denmark.
出版信息
Forensic Sci Int. 1994 Feb;64(2-3):83-8. doi: 10.1016/0379-0738(94)90216-x.
PMID:8175092
Abstract
A method describing determination of the physiological ketone bodies: acetone, acetoacetate, and D-beta-hydroxybutyrate in postmortem blood by head-space gas chromatography. The method involves enzymic dehydrogenation of D-beta-hydroxybutyrate into acetoacetate and quantitative conversion of acetoacetate into acetone. The recoveries of acetone, acetoacetate and D-beta-hydroxybutyrate were 94-100%, 88-104% and 83-91%, respectively.
摘要
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