Chemistry Department, Université du Québec à Montréal (UQÀM), Montréal, Québec, Canada H3C 3P8.
J Anal Toxicol. 2010 Nov;34(9):562-70. doi: 10.1093/jat/34.9.562.
Beryllium (Be) is still not well understood from a toxicological point of view, and studies that involve the determination of different Be compounds species in tissues need to be conducted. In this paper we describe the development and validation of reliable methods for the detection of ultra-trace levels of Be in various biological matrices. Blood and tissues (liver, lung, spleen, and kidney) were used in this study. The samples were digested with a mixture of nitric and perchloric acids for Be and BeAl and an addition of sulfuric acid was made for BeO. The solutions were analyzed by inductively coupled plasma mass spectrometry with (6)Li as internal standard. The detection limits are in the order of 0.02 ng/g for tissue and 0.03 ng/mL for blood, and were compared to existing reference methods. To our knowledge, this is the first study that assesses dissolution of the different Be compounds in biological matrices, while also undergoing a rigorous optimization and complete validation. This method has proven that it is reliable, among the most sensitive available in the literature, and that it can be used in trace toxicological studies for Be.
铍（Be）从毒理学角度来看仍未被充分了解，需要进行涉及组织中不同铍化合物形态测定的研究。本文描述了开发和验证可靠方法的过程，用于检测各种生物基质中超痕量水平的铍。本研究使用了血液和组织（肝、肺、脾和肾）。样品用硝酸和高氯酸的混合物消解用于 Be 和 BeAl，并且添加了硫酸用于 BeO。采用电感耦合等离子体质谱法（ICP-MS），以 6Li 作为内标进行分析。检测限为组织中的 0.02 ng/g 左右，血液中的 0.03 ng/mL 左右，并与现有参考方法进行了比较。据我们所知，这是首次评估不同铍化合物在生物基质中的溶解情况的研究，同时也进行了严格的优化和全面验证。该方法已被证明可靠，是文献中最敏感的方法之一，可用于铍的痕量毒理学研究。
