Nakamura S, Oda Y, Ugawa M
Osaka Prefectural Institute of Public Health, Japan.
Mutat Res. 1990 Mar;229(1):11-5. doi: 10.1016/0027-5107(90)90003-m.
The genotoxicity of dimethyl sulfoxide (DMSO) was demonstrated by the umu test using Salmonella typhimurium TA1535/pSK1002 carrying the umuC-lacZ fusion gene. The level of beta-galactosidase activity which shows umu gene expression in the test system was dependent on the concentration of DMSO in the culture medium. The maximum beta-galactosidase activity was approximately 3.5 times as high as the background level with 10% of DMSO in the culture medium. The lowest concentration of DMSO required for a response of over twice the background level was approximately 5%. Four structurally related chemicals (acetone, di-n-butylsulfoxide, dimethylsulfide, methylphenylsulfoxide) did not show umu gene expression at their non-toxic doses.
使用携带umuC-lacZ融合基因的鼠伤寒沙门氏菌TA1535/pSK1002通过umu试验证明了二甲基亚砜（DMSO）的遗传毒性。在测试系统中显示umu基因表达的β-半乳糖苷酶活性水平取决于培养基中DMSO的浓度。当培养基中含有10%的DMSO时，最大β-半乳糖苷酶活性约为背景水平的3.5倍。产生超过背景水平两倍响应所需的DMSO最低浓度约为5%。四种结构相关的化学物质（丙酮、二正丁基亚砜、二甲基硫醚、甲基苯基亚砜）在其无毒剂量下未显示umu基因表达。
