Schmid C, Reifferscheid G, Zahn R K, Bachmann M
Institut für Physiologische Chemie, Johannes Gutenberg Universität Mainz, Germany.
Mutat Res. 1997 Nov 27;394(1-3):9-16. doi: 10.1016/s1383-5718(97)00118-6.
The SOS/umu-test with Salmonella typhimurium TA1535/pSK1002 as tester strain is a rapid and valuable bacterial assay for screening of umuC-dependent mutagenic potential of chemical compounds and chemicals relevant to environmental pollution. The initial assay was modified by replacing the beta-galactosidase reporter gene with luciferase. Thereby, the sensitivity of the umu-test was increased significantly and the susceptibility to intensively coloured solutions was reduced. The alternative enzyme assay in the modified umu-test (umu-Luc) represents an independent method which allows to confirm the colorimetric results obtained with the original SOS/umu-test system (umu-Gal) by measuring the beta-galactosidase activity. It has been proved that for several genotoxins an increased induction rate could be achieved by applying the new umu-Luc test.
以鼠伤寒沙门氏菌TA1535/pSK1002作为测试菌株的SOS/umu试验，是一种快速且有价值的细菌检测方法，用于筛选与环境污染相关的化合物和化学物质的umuC依赖性诱变潜力。最初的试验通过用荧光素酶替代β-半乳糖苷酶报告基因进行了改进。由此，umu试验的灵敏度显著提高，对颜色较深溶液的敏感性降低。改进后的umu试验（umu-Luc）中的替代酶检测代表了一种独立的方法，通过测量β-半乳糖苷酶活性，可以确认使用原始SOS/umu试验系统（umu-Gal）获得的比色结果。事实证明，对于几种基因毒素，应用新的umu-Luc试验可以提高诱导率。
