Fertility Clinic and IVF Center, Mumbai, India.
Fertil Steril. 2011 Feb;95(2):631-4. doi: 10.1016/j.fertnstert.2010.10.007. Epub 2010 Nov 12.
To compare fertilization, cleavage, and pregnancy outcome using pentoxifylline and a hypoosmotic swelling (HOS) test to select viable spermatozoa from testicular biopsy specimens.
Open, comparative, prospective study.
G.S. Medical College and Fertility clinic, Mumbai, India.
PATIENT(S): A total of 50 couples enrolled for infertility treatment having a male factor indication of nonobstructive azoospermia.
INTERVENTION(S): Assessment of viable spermatozoa using pentoxifylline and using an HOS test from a population of nonmotile spermatozoa obtained from testicular biopsies.
MAIN OUTCOME MEASURE(S): Comparison of fertilization, cleavage, and clinical pregnancy rates using viable sperms recovered using pentoxifylline and an HOS test.
RESULT(S): Viable spermatozoa were obtained in both the study groups. Significantly higher fertilization rates (pentoxifylline 62.05% vs. HOS 41.07%) and clinical pregnancy rates (pentoxifylline 32% vs. HOS 16%) were observed. There was no significant difference in cleavage rates among both groups.
CONCLUSION(S): We found that obtaining viable spermatozoa using pentoxifylline was more effective in terms of fertilization and pregnancies than obtaining it through an HOS test.
比较使用己酮可可碱和低渗肿胀（HOS）试验选择从睾丸活检标本中获取有活力精子的受精、卵裂和妊娠结局。
开放、对比、前瞻性研究。
印度孟买 G.S. 医学院和生育诊所。
共有 50 对夫妇因男性因素导致非梗阻性无精子症而接受不孕治疗。
使用己酮可可碱和 HOS 试验评估从睾丸活检中获得的非运动精子中的有活力精子。
比较使用己酮可可碱和 HOS 试验回收的有活力精子的受精、卵裂和临床妊娠率。
在两个研究组中均获得了有活力的精子。观察到受精率（己酮可可碱 62.05%比 HOS 41.07%）和临床妊娠率（己酮可可碱 32%比 HOS 16%）有显著提高。两组的卵裂率无显著差异。
我们发现，与 HOS 试验相比，使用己酮可可碱获取有活力的精子在受精和妊娠方面更有效。
