Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC.
Colloids Surf B Biointerfaces. 2011 Feb 1;82(2):616-23. doi: 10.1016/j.colsurfb.2010.10.031. Epub 2010 Oct 23.
A uniform de novo production of neocartilage is a critical issue in the fabrication of tissue-engineered diarthrodial substitutes. The aim of this work is to develop homogeneous chondrogenesis in heparinized scaffolds with pores of inverted colloidal crystal (ICC) geometry. Monodispersed polystyrene microspheres were self-assembled by floating in the medium containing ethylene glycol, dried, annealed and infiltrated with heparin/chitin/chitosan gels. The results indicated that the colloidal template was in a structure of hexagonal arrays. In addition, the regularity of the organized pores in the scaffolds reduced when the concentration of ethylene glycol decreased. An increase in the weight percentage of heparin enhanced the viability of bovine knee chondrocytes (BKCs) in ICC matrices. Over 4 weeks of cultivation, the amount of cartilaginous components including BKCs, glycosaminoglycans (GAGs) and collagen enhanced with time. Moreover, an increase in the weight percentage of heparin promoted the production of BKCs, GAGs and collagen in ICC constructs. Histological and immunochemical staining of the cultured ICC constructs revealed minor differences in BKCs, GAGs and type II collagen between the peripheral and core regions. Therefore, the ordered pores in the heparinized ICC constructs could favor the chondrocyte culture to regenerate a uniform distribution of cartilage.
在组织工程化关节替代物的制造中，均一的新生软骨是一个关键问题。本工作旨在开发肝素化支架中具有反胶束晶体（ICC）几何形状的孔的同质软骨发生。单分散聚苯乙烯微球通过在含有乙二醇的介质中浮起来自组装，然后干燥、退火并渗透肝素/壳聚糖/壳聚糖凝胶。结果表明胶体模板为六方排列结构。此外，当乙二醇浓度降低时，支架中有序孔的规律性降低。肝素重量百分比的增加增强了 ICC 基质中牛膝关节软骨细胞（BKCs）的活力。在培养的 4 周内，包括 BKCs、糖胺聚糖（GAGs）和胶原蛋白在内的软骨成分的数量随时间增加。此外，肝素重量百分比的增加促进了 ICC 构建体中 BKCs、GAGs 和胶原蛋白的产生。培养的 ICC 构建体的组织学和免疫化学染色显示，在周边和核心区域之间，BKCs、GAGs 和 II 型胶原蛋白的差异较小。因此，肝素化 ICC 构建体中的有序孔有利于软骨细胞培养再生均匀分布的软骨。
