CIVEN, via delle Industrie 5, 30175 Marghera, Venezia, Italy.
Biosens Bioelectron. 2011 Jan 15;26(5):2761-5. doi: 10.1016/j.bios.2010.10.024. Epub 2010 Oct 21.
DNA microarray is a powerful tool for the parallel of nucleic acids and other biologically significant molecules. In this communication we report an easy and cheap synthesis route for incorporating organic dyes into monodisperse inorganic silica nanoparticles and their application on the detection of carcinogenic risky Human Papilloma Virus using DNA microarray technology. We correlate our system with conventional direct dyes and commercial quantum dots, with a promising increase in optical signal, and a related decrease of the limit of detection, thus giving a remarkable improvement in this technique towards early diagnosis of diseases and trace level detection of dangerous biological contaminants.
DNA 微阵列是一种用于平行核酸和其他具有生物学意义的分子的强大工具。在本通讯中，我们报告了一种简便、廉价的合成路线，用于将有机染料掺入单分散无机硅纳米粒子中，并将其应用于基于 DNA 微阵列技术检测致癌风险的人类乳头瘤病毒。我们将我们的系统与传统的直接染料和商业量子点进行了比较，光学信号有了明显的提高，检测限也相应降低，因此，该技术在疾病的早期诊断和危险生物污染物的痕量检测方面有了显著的改进。
