Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, South Korea.
Dev Comp Immunol. 2011 Mar;35(3):258-62. doi: 10.1016/j.dci.2010.11.004. Epub 2010 Nov 21.
Members of the tumor necrosis factor (TNF) and TNF receptor (TNFR) superfamilies play crucial roles in both innate and adaptive immunity. In the present study, we isolated the full-length cDNA for black rockfish (Sebastes schlegelii) TNFR (BrTNFR). This cDNA is 2405 bp in length and contains a 939-bp open reading frame, a 27-bp 5' untranslated region, and a 1439-bp 3' untranslated region including a polyadenylation signal (AATAAA) and polyadenylation site. The 313-amino-acid predicted BrTNFR sequence is homologous to other TNFR sequences, contains four cysteine-rich domains and a death-effector domain (DED), and lacks a transmembrane region. Expression of BrTNFR mRNA was detected in eight different tissues from healthy black rockfish and was highest in peripheral blood lymphocytes and gills. In analyses of mitogen-stimulated BrTNFR expression in peripheral blood lymphocytes, expression of BrTNFR mRNA was observed between 1 and 24h after stimulation with lipopolysaccharide, concanavalin A/phorbol myristate acetate, or poly I:C. Although the data suggest that BrTNFR represents an ancestral member of the TNFR superfamily, the orthology of TNFR in teleost fish is difficult to establish because few TNFRs have been identified in these species.
肿瘤坏死因子（TNF）和 TNF 受体（TNFR）超家族成员在先天和适应性免疫中都起着至关重要的作用。在本研究中，我们分离了黑鲷（Sebastes schlegelii）TNFR（BrTNFR）的全长 cDNA。该 cDNA 长 2405bp，包含一个 939bp 的开放阅读框、一个 27bp 的 5'非翻译区和一个 1439bp 的 3'非翻译区，包括一个多聚腺苷酸化信号（AATAAA）和多聚腺苷酸化位点。预测的 BrTNFR 序列由 313 个氨基酸组成，与其他 TNFR 序列同源，含有四个富含半胱氨酸的结构域和一个死亡效应结构域（DED），但没有跨膜区。在健康黑鲷的八个不同组织中检测到 BrTNFR mRNA 的表达，在外周血淋巴细胞和鳃中表达最高。在分析外周血淋巴细胞中 BrTNFR 表达的有丝分裂原刺激时，在 LPS、刀豆蛋白 A/佛波醇乙酸酯或聚 I:C 刺激后 1 至 24 小时观察到 BrTNFR mRNA 的表达。尽管数据表明 BrTNFR 代表 TNFR 超家族的一个古老成员，但由于在这些物种中鉴定出的 TNFR 很少，因此很难确定鱼类 TNFR 的同源性。
