Institut des Régions Arides, Médenine, Tunisia.
Food Chem Toxicol. 2011 Feb;49(2):342-7. doi: 10.1016/j.fct.2010.11.003. Epub 2010 Nov 12.
The essential oil of Artemisia campestris and the ethanol-water, hexane and water extracts of A. campestris and Thymelaea hirsuta collected in southern of Tunisia were investigated for their antioxidant (DPPH, ABTS and beta-carotene methods) and antitumor growth inhibition of human colon cancer HT-29 cells using MTT test activities. All the A. campestris extracts tested at high concentrations (100 μg/ml) showed activity ranging from 19.5% for essential oil to 64.4% of negative control growth for infusion extract, except the hexane extract. With T. hirsuta, all the extracts tested (hexane and ethanol-water), except the infusion extract, also exhibited antitumor activity (58.2% and 65.5% of control growth respectively). The ethanol-water and infusion extracts of A. campestris showed higher antioxidant activity, polyphenol and flavonoid contents than those of T. hirsuta. These results show that there is a positive correlation between the antitumor activity and the antioxidant activity, and of these two activities and with the levels of polyphenols and flavonoids. The essential oil and the other extracts of A. campestris, which exhibited significant antitumor activity against the HT-29 cells deserve further research into the chemoprevention and treatment of colon cancer.
研究了在突尼斯南部采集的艾草精油和艾草及毛瑞香的乙醇水、正己烷和水提取物的抗氧化（DPPH、ABTS 和β-胡萝卜素法）和抑制人结肠癌 HT-29 细胞生长的抗肿瘤活性，用 MTT 试验进行检测。所有在高浓度（100 μg/ml）下测试的艾草提取物（从精油的 19.5%到浸膏提取物的 64.4%）都表现出活性，除了正己烷提取物。对于毛瑞香，所有测试的提取物（正己烷和乙醇水提取物），除了浸膏提取物，也表现出抗肿瘤活性（分别为对照生长的 58.2%和 65.5%）。艾草的乙醇水和浸膏提取物表现出比毛瑞香更高的抗氧化活性、多酚和类黄酮含量。这些结果表明，抗肿瘤活性和抗氧化活性之间存在正相关，而且这两种活性与多酚和类黄酮的水平之间存在正相关。具有显著抗肿瘤活性的艾草精油和其他提取物值得进一步研究，以预防和治疗结肠癌。
