Immunology Research Center, BuAli Research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Razavi Khorasan Province, Iran.
Pharm Biol. 2011 Sep;49(9):962-9. doi: 10.3109/13880209.2011.559251. Epub 2011 May 19.
Different species of Artemisia (Asteraceae) have shown to exhibit antitumor activity. The aim of this study was to identify the antiproliferative effect of some Artemisia species from Iran on cultured human cancer cells.
Methanol, ethyl acetate, dichloromethane and n-hexane extracts from aerial parts of seven species of Artemisia were prepared and their antiproliferative effects on four cancer (AGS, HeLa, HT-29 and MCF-7) and normal cell line (L929) were determined. Different concentrations of extracts were added to cultured cells and incubated for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was employed to assess the cell viability.
Different extracts exert various growth inhibitory effects. In case of AGS cells, dichloromethane and methanol extracts of A. ciniformis Krasch. & Popov ex Poljak. (IC(50) values: 35 and 60 µg/ml, respectively) showed the highest growth inhibitory effects. HeLa cells were more sensitive to both A. diffusa Krasch. ex Poljak. dichloromethane (IC(50) value: 71 µg/ml) and A. ciniformis ethylacetate (IC(50) value: 73 µg/ml) extracts. Dichloromethane extracts of A. diffusa, A. santolina Schrenk and A. ciniformis (IC(50) values: 42, 91 and 94 µg/ml, respectively) exhibited more inhibition on HT-29 cells in comparison to other extracts. MCF-7 cells were best inhibited by A. ciniformis dichloromethane (IC(50) value: 29 µg/ml) and A. vulgaris L. ethyl acetate (IC(50) value: 57 µg/ml) extracts.
This study shows the antiproliferative effects of Artemisia extracts on malignant cell lines. Artemisia could be also considered as a promising chemotherapeutic agent in cancer treatment.
不同种的蒿属（菊科）植物已显示出抗肿瘤活性。本研究旨在鉴定来自伊朗的一些蒿属植物对培养的人类癌细胞的增殖抑制作用。
用甲醇、乙酸乙酯、二氯甲烷和正己烷从 7 种蒿属植物的地上部分制备提取物，并测定其对 4 种癌细胞（AGS、HeLa、HT-29 和 MCF-7）和正常细胞系（L929）的增殖抑制作用。将不同浓度的提取物加入培养细胞中，孵育 72 小时。采用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法评估细胞活力。
不同提取物表现出不同的生长抑制作用。AGS 细胞中，A. ciniformis Krasch. & Popov ex Poljak. 的二氯甲烷和甲醇提取物（IC50 值分别为 35 和 60μg/ml）表现出最强的生长抑制作用。HeLa 细胞对 A. diffusa Krasch. ex Poljak. 的二氯甲烷（IC50 值：71μg/ml）和 A. ciniformis 乙酸乙酯提取物（IC50 值：73μg/ml）均更为敏感。与其他提取物相比，A. diffusa、A. santolina Schrenk 和 A. ciniformis 的二氯甲烷提取物（IC50 值分别为 42、91 和 94μg/ml）对 HT-29 细胞的抑制作用更强。MCF-7 细胞受 A. ciniformis 二氯甲烷（IC50 值：29μg/ml）和 A. vulgaris L. 乙酸乙酯提取物（IC50 值：57μg/ml）的抑制作用最强。
本研究表明蒿属植物提取物对恶性细胞系具有增殖抑制作用。蒿属植物也可被视为癌症治疗中有前途的化疗药物。
