BD GeneOhm 耐甲氧西林金黄色葡萄球菌（MRSA）PCR 检测法用于检测台湾成年人 MRSA 鼻腔定植的性能。
Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay for detecting MRSA nasal colonization in Taiwanese adults.
机构信息
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
出版信息
J Microbiol Immunol Infect. 2010 Oct;43(5):372-7. doi: 10.1016/S1684-1182(10)60059-X.
BACKGROUND/PURPOSE: A rapid diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) has been implemented for surveillance of the at-risk population, but its performance in those without traditional risk factors is not clear. The objective of this study was to evaluate MRSA colonization status by comparing the performance of the BD GeneOhm MRSA polymerase chain reaction (PCR) assay with that of conventional culture during a 3-month active surveillance of Taiwanese adults in the community.
METHODS
From 1 October 2007 to 28 December 2007, adults (≥ 18 years old) attending a mandatory health examination arranged by their employers as a part of the workplace health promotion program at three medical centers in northern Taiwan were enrolled in the study. No healthcare workers were included. A total of 498 paired nasal swabs were prospectively obtained and used for both the BD GeneOhm MRSA PCR assay and conventional culture.
RESULTS
Of the 498 paired nasal swabs, 14 (2.8%) were positive for MRSA by conventional culture and 34 (6.8%) were positive by the BD GeneOhm MRSA PCR assay (p < 0.005). Thirteen specimens were both culture- and PCR-positive, and 463 samples were both culture- and PCR-negative. There were two discordant results: 21 specimens were culture-negative/PCR-positive, and one was culture-positive/PCR-negative. The simple kappa coefficient for measuring the agreement between conventional culture and the MRSA PCR assay was 0.52.
CONCLUSION
This study demonstrates the feasibility of using both the MRSA PCR assay and conventional culture as surveillance tools. Also, the MRSA-positive rate detected by MRSA PCR assay was significantly higher than that of conventional culture.
背景/目的：已经实施了一种用于检测耐甲氧西林金黄色葡萄球菌（MRSA）的快速诊断方法，用于监测高危人群，但在没有传统危险因素的人群中的表现尚不清楚。本研究的目的是通过比较 BD GeneOhm MRSA 聚合酶链反应（PCR）检测与传统培养在台湾社区成年人群中进行的 3 个月主动监测中的表现，来评估 MRSA 定植状态。
方法
从 2007 年 10 月 1 日至 2007 年 12 月 28 日，参加台湾北部三个医疗中心作为工作场所健康促进计划的一部分安排的强制性健康检查的成年人（≥18 岁）被纳入研究。不包括医护人员。前瞻性地获得了 498 对鼻拭子，分别用于 BD GeneOhm MRSA PCR 检测和传统培养。
结果
在 498 对鼻拭子中，有 14 份（2.8%）经传统培养阳性为 MRSA，34 份（6.8%）经 BD GeneOhm MRSA PCR 检测阳性（p<0.005）。13 份标本均为培养和 PCR 阳性，463 份标本均为培养和 PCR 阴性。有两个不一致的结果：21 份标本培养阴性/PCR 阳性，1 份标本培养阳性/PCR 阴性。用于测量传统培养与 MRSA PCR 检测之间一致性的简单 Kappa 系数为 0.52。
结论
本研究表明，MRSA PCR 检测和传统培养均可作为监测工具。此外，MRSA PCR 检测检测到的 MRSA 阳性率明显高于传统培养。
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