Department of Pathology and Laboratory Medicine, University of Louisville Hospital, Louisville, KY 40202, USA.
J Clin Microbiol. 2010 Apr;48(4):1305-9. doi: 10.1128/JCM.01326-09. Epub 2010 Feb 24.
This study compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) real-time PCR assay to culture by the use of BBL CHROMagar MRSA for the detection of MRSA in 627 nasal surveillance specimens collected from intensive care unit (ICU) patients. The PCR assay had a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 96.7%, 70.3%, and 100%, respectively. Nine of 19 false-positive PCR specimens grew methicillin-susceptible S. aureus (MSSA) from broth enrichment culture, of which two demonstrated evidence of mecA gene dropout. Compared to culture by the use of BBL CHROMagar MRSA, the BD GeneOhm MRSA PCR assay demonstrated sensitivity and specificity above 95% for the detection of MRSA nasal colonization and provided shorter turnaround time in generating positive and negative final results.
本研究比较了 BD GeneOhm 耐甲氧西林金黄色葡萄球菌（MRSA）实时 PCR 检测法与 BBL CHROMagar MRSA 培养法，用于检测从重症监护病房（ICU）患者采集的 627 份鼻腔监测标本中的 MRSA。PCR 检测法的灵敏度、特异性、阳性预测值和阴性预测值分别为 100%、96.7%、70.3%和 100%。19 份假阳性 PCR 标本中有 9 份从肉汤增菌培养中生长出对甲氧西林敏感的金黄色葡萄球菌（MSSA），其中 2 份显示 mecA 基因缺失的证据。与 BBL CHROMagar MRSA 培养法相比，BD GeneOhm MRSA PCR 检测法对检测 MRSA 鼻腔定植的灵敏度和特异性均高于 95%，并在生成阳性和阴性最终结果时提供了更短的周转时间。
