Cao Meng, Takaoka Akio, Zhang Hai-Bo, Nishi Ryuji
Key Laboratory for Physical Electronics and Devices of the Ministry of Education, Department of Electronic Science and Technology, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.
J Electron Microsc (Tokyo). 2011;60(1):39-46. doi: 10.1093/jmicro/dfq076. Epub 2010 Nov 12.
We presented an automatic method for detecting and tracking colloidal gold fiducial markers for alignment in electron tomography (ET). The second-order derivative of direction was used to detect a fiducial marker accurately. The detection was optimized to be selective to the size of fiducial markers. A preliminary tracking result from the normalized correlation coefficient was refined using the detector. A constraint model considering the relationship among the fiducial markers on different images was developed for removing outlier. The three-dimensional positions of the detected fiducial markers and the projection parameters of tilt images were calculated for post process. The accuracy of detection and tracking results was evaluated from the residues by the software IMOD. Application on transmission electron microscopic images also indicated that the presented method could provide a useful approach to automatic alignment in ET.
我们提出了一种用于在电子断层扫描（ET）中检测和跟踪胶体金基准标记以进行对齐的自动方法。利用方向的二阶导数来精确检测基准标记。该检测经过优化，对基准标记的大小具有选择性。使用检测器对归一化相关系数的初步跟踪结果进行细化。开发了一个考虑不同图像上基准标记之间关系的约束模型以去除异常值。计算检测到的基准标记的三维位置和倾斜图像的投影参数以进行后处理。通过软件IMOD根据残差评估检测和跟踪结果的准确性。在透射电子显微镜图像上的应用也表明，所提出的方法可为ET中的自动对齐提供一种有用的方法。
