Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
College of Life Sciences, Hebei University, Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, PR China.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2425-2429. doi: 10.1099/ijs.0.026146-0. Epub 2010 Nov 12.
A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127(T), was isolated from the roots of fresh rice plants (Oryza sativa). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium oryzae Alt 505(T) (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the gyrB, atpD, recA and glnII gene sequences of strain J3-A127(T) and the type strains of recognized species of the genus Rhizobium also indicated that it represented a separate species. The temperature range for growth was 10-40 °C (optimum around 28 °C) and the pH range was 6.0-11.0 (optimum pH 7.0-8.0). Strain J3-A127(T) tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6; 46.7 %). The DNA G+C content of strain J3-A127(T) was 59.5 mol%. Strain J3-A127(T) did not form any nodules on four different legumes and the nodD and nifH genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pseudoryzae sp. nov. is proposed. The type strain is J3-A127(T) ( = ACCC 10380(T) = KCTC 23294(T)).
一株革兰氏阴性、需氧、杆状细菌，被命名为 J3-A127(T) 菌株，从新鲜水稻植株（Oryza sativa）的根部分离得到。细胞是非运动性的，未检测到鞭毛。16S rRNA 基因序列的比较表明，该菌株与根瘤菌属的种具有系统发育关系，与 Rhizobium oryzae Alt 505(T)（96.4%）最为相似。J3-A127(T) 菌株的 16S rRNA 基因序列与公认的根瘤菌属种的 gyrB、atpD、recA 和 glnII 基因序列之间的相似性较低（<90%），这也表明它代表了一个单独的种。该菌株的生长温度范围为 10-40°C（最佳温度约为 28°C），pH 值范围为 6.0-11.0（最佳 pH 值为 7.0-8.0）。菌株 J3-A127(T) 能耐受高达 5.0%（w/v）的 NaCl 浓度。该菌株过氧化氢酶和氧化酶均为阳性。主要的细胞脂肪酸为 sumed feature 8（C(18:1)ω7c 和/或 C(18:1)ω6；46.7%）。菌株 J3-A127(T) 的 DNA G+C 含量为 59.5 mol%。J3-A127(T) 菌株在四种不同的豆科植物上均未形成任何根瘤，且 PCR 未检测到 nodD 和 nifH 基因。根据生理生化特征和基因型数据，菌株 J3-A127(T) 被认为代表了根瘤菌属的一个新种，建议将其命名为 Rhizobium pseudoryzae sp. nov.。其模式菌株为 J3-A127(T)（ = ACCC 10380(T) = KCTC 23294(T)）。
