Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Department of Soil Science and Soil Water Conservation, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1373-1377. doi: 10.1099/ijs.0.056325-0. Epub 2014 Jan 21.
Two strains (J3-AN59(T) and J3-N84) of Gram-stain-negative, aerobic and rod-shaped bacteria were isolated from the roots of fresh rice plants. The 16S rRNA gene sequence similarity results showed that the similarity between strains J3-AN59(T) and J3-N84 was 100 %. Both strains were phylogenetically related to members of the genus Rhizobium, and they were most closely related to Rhizobium tarimense ACCC 06128(T) (97.43 %). Similarities in the sequences of housekeeping genes between strains J3-AN59(T) and J3-N84 and those of recognized species of the genus Rhizobium were less than 90 %. The polar lipid profiles of both strains were predominantly composed of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The DNA G+C contents of J3-AN59(T) and J3-N84 were 55.7 and 57.1 mol%, respectively. The DNA-DNA relatedness value between J3-AN59(T) and J3-N84 was 89 %, and strain J3-AN59(T) showed 9 % DNA-DNA relatedness to R. tarimense ACCC 06128(T), the most closely related strain. Based on this evidence, we found that J3-AN59(T) and J3-N84 represent a novel species in the genus Rhizobium and we propose the name Rhizobium rhizoryzae sp. nov. The type strain is J3-AN59(T) ( = ACCC 05916(T) = KCTC 23652(T)).
两株革兰氏阴性、需氧、杆状细菌（J3-AN59(T) 和 J3-N84）从新鲜水稻根部分离得到。16S rRNA 基因序列相似性结果表明，菌株 J3-AN59(T) 和 J3-N84 之间的相似度为 100%。两株菌均与根瘤菌属的成员具有系统发育关系，与塔里木盐单胞菌 ACCC 06128(T)（97.43%）最为密切相关。菌株 J3-AN59(T) 和 J3-N84 与公认的根瘤菌属种之间的看家基因序列相似性均小于 90%。两株菌的极性脂图谱主要由磷脂酰甘油、双磷脂酰甘油、磷脂乙醇胺、磷脂胆碱和一种未知的氨基磷脂组成。主要的细胞脂肪酸为 summed feature 8（C18:1ω7c 和/或 C18:1ω6c）和 C16:0。J3-AN59(T) 和 J3-N84 的 DNA G+C 含量分别为 55.7mol%和 57.1mol%。J3-AN59(T) 和 J3-N84 之间的 DNA-DNA 同源性为 89%，而菌株 J3-AN59(T) 与最密切相关的菌株塔里木盐单胞菌 ACCC 06128(T) 的 DNA-DNA 同源性为 9%。基于这些证据，我们发现 J3-AN59(T) 和 J3-N84 代表根瘤菌属中的一个新种，我们提议将其命名为 Rhizobium rhizoryzae sp. nov. 。模式菌株为 J3-AN59(T)（=ACCC 05916(T)=KCTC 23652(T)）。
