Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2962-2968. doi: 10.1099/ijs.0.062117-0. Epub 2014 Jun 4.
An aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain CC-LY845(T), was isolated from the surface of rice straw in Taiwan. Cells were non-motile, and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium pseudoryzae KCTC 23294(T) (97.6 %), R. rhizoryzae KCTC 23652(T) (97.0 %) and R. oryzae LMG 24253(T) (96.7 %); other species showed lower levels of similarity (<96.6 %). The DNA-DNA relatedness of strain CC-LY845(T) and R. pseudoryzae KCTC 23294(T) was 34.8±3.1 % (reciprocal value 39.2±2.2 %). Phylogenetic analysis based on the housekeeping atpD and recA genes showed that the novel strain could be distinguished from R. pseudoryzae KCTC 23294(T) (92.7 and 91.5 %, respectively) and other species of the genus Rhizobium. The temperature range for growth was 25-42 °C, the pH range was 5.0-9.0 and NaCl concentrations up to 4.0 % (w/v) were tolerated. Strain CC-LY845(T) did not form nodules on four different legumes, and the nodD and nifH genes were not detected by PCR. The major fatty acids were C16 : 0 and summed feature 8 (C18 : 1ω7c/C18 : 1ω6c). The polyamine pattern of strain CC-LY845(T) showed spermidine and putrescine as major polyamines. The predominant quinone system was ubiquinone 10 (Q-10). The DNA G+C content was 68.3±2.4 mol%. Base on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY845(T) is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium straminoryzae sp. nov. is proposed. The type strain is strain CC-LY845(T) ( = BCRC 80698(T) = JCM 19536(T)).
一株好氧、革兰氏阴性、杆状细菌，被命名为 CC-LY845(T) 菌株，从台湾稻草表面分离得到。细胞是非运动的，没有发现鞭毛。16S rRNA 基因序列比较表明，该菌株与根瘤菌属的种亲缘关系最密切，与 Rhizobium pseudoryzae KCTC 23294(T)（97.6%）、R. rhizoryzae KCTC 23652(T)（97.0%）和 R. oryzae LMG 24253(T)（96.7%）最为相似；其他种的相似性较低（<96.6%）。菌株 CC-LY845(T) 和 Rhizobium pseudoryzae KCTC 23294(T) 的 DNA-DNA 同源性为 34.8±3.1%（倒数为 39.2±2.2%）。基于看家基因 atpD 和 recA 基因的系统发育分析表明，该新菌株可与 Rhizobium pseudoryzae KCTC 23294(T)（分别为 92.7%和 91.5%）和根瘤菌属的其他种区分开来。生长的温度范围为 25-42°C，pH 值范围为 5.0-9.0，可耐受 4.0%（w/v）的 NaCl 浓度。CC-LY845(T) 菌株不能在四种不同的豆科植物上形成根瘤，PCR 也未检测到 nodD 和 nifH 基因。主要脂肪酸为 C16：0 和总和特征 8（C18：1ω7c/C18：1ω6c）。菌株 CC-LY845(T) 的聚胺图谱显示腐胺和亚精胺为主要聚胺。主要的醌类系统是泛醌 10（Q-10）。DNA G+C 含量为 68.3±2.4 mol%。基于其系统发育、表型和化学分类特征，菌株 CC-LY845(T) 代表根瘤菌属中的一个新种，建议命名为 Rhizobium straminoryzae sp. nov.。模式菌株为 CC-LY845(T) 菌株（=BCRC 80698(T)=JCM 19536(T)）。
