Key Laboratory of Tropical Microbial Resources, Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, PR China.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):241-248. doi: 10.1099/ijs.0.038232-0. Epub 2012 Mar 2.
Three novel actinobacteria, strains 39(T), 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39(T) represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9 % 16S rRNA gene sequence similarity), Branchiibius (95.7 %), Dermacoccus (94.4-95.3 %), Calidifontibacter (94.6 %), Luteipulveratus (94.3 %), Yimella (94.2 %) and Kytococcus (93.1 %). Cells were irregular cocci and short rods. The peptidoglycan type was A4α with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C(17 : 0) (41.97 %), anteiso-C(17 : 1)ω9c (32.16 %) and iso-C(16 : 0) (7.68 %). The DNA G+C content of strain 39(T) was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39(T) (=CGMCC 4.6864(T) = DSM 24617(T)).
从南极巴伦蒂亚斯岛采集的土壤中分离到三株新的放线菌，菌株 39(T)、40 和 41。使用多相方法确定这些菌株的分类地位。16S rRNA 基因序列比较表明，菌株 39(T)代表了皮科家族的一个新谱系，与属代美西亚（96.9% 16S rRNA 基因序列相似性）、Branchiibius（95.7%）、Dermacoccus（94.4-95.3%）、Calidifontibacter（94.6%）、Luteipulveratus（94.3%）、Yimella（94.2%）和 Kytococcus（93.1%）的成员最为密切相关。细胞呈不规则球菌和短杆菌。肽聚糖类型为 A4α，具有 L-Lys-L-Ser-D-Asp 间肽桥。细胞壁糖为半乳糖和葡萄糖。主要的menaquinone 是 MK-8(H4)。极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷酸糖脂、两种糖脂和一种未知的磷脂。细胞壁多糖的酰基类型为 N-乙酰。主要细胞脂肪酸为 anteiso-C(17:0)（41.97%）、anteiso-C(17:1)ω9c（32.16%）和 iso-C(16:0)（7.68%）。菌株 39(T)的 DNA G+C 含量为 68.4mol%。基于与皮科家族其他属的系统发育和表型差异，提出了一个新的属和种，巴伦蒂亚斯imonas humi 属。nov.，sp. nov.，其模式种为 39(T)=CGMCC 4.6864(T)=DSM 24617(T)。
