Departamento de Microbiología y Parasitología, Facultad de Biología. Universidad de Santiago de Compostela. 15782, Santiago de Compostela, Spain.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ghent, Belgium.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2406-2411. doi: 10.1099/ijs.0.025320-0. Epub 2010 Nov 12.
Facultatively anaerobic marine bacteria isolated from cultured clams, Ruditapes decussatus and Ruditapes philippinarum, were previously investigated using amplified fragment length polymorphism (AFLP) and 16S rRNA gene sequence analyses. The isolates formed two AFLP clusters and belonged to the genus Vibrio, more precisely to the Splendidus clade. In this study, phylogenetic analyses based on sequences of the housekeeping genes rpoA, rpoD, pyrH, atpA and recA supported their inclusion in that clade forming two well differentiated groups with respect to the rest of the species within the clade, and confirmed that they formed two groups, separated from the rest of the species of the clade. DNA-DNA hybridization demonstrated that the isolates constitute two novel species of the genus Vibrio, which can be phenotypically differentiated from their closest relatives. The names Vibrio atlanticus sp. nov. and Vibrio artabrorum sp. nov. are proposed, with Vb 11.11(T) ( = CECT 7223(T) = LMG 24300(T)) and Vb 11.8(T) ( = CECT 7226(T) = LMG 23865(T)) as the type strains, respectively.
从养殖的蛤类，即菲律宾蛤仔和文蛤中分离到的兼性厌氧海洋细菌，先前已经使用扩增片段长度多态性（AFLP）和 16S rRNA 基因序列分析进行了研究。这些分离株形成了两个 AFLP 聚类，属于弧菌属，更确切地说是辉煌弧菌群。在本研究中，基于看家基因 rpoA、rpoD、pyrH、atpA 和 recA 的序列进行的系统发育分析支持将它们归入该分支，在该分支内的其余物种形成两个分化良好的组，并证实它们与该分支内的其余物种形成两个组。DNA-DNA 杂交表明，这些分离株构成了弧菌属的两个新种，它们可以从与其最接近的亲缘种表型上区分开来。提议使用 Vibrio atlanticus sp. nov. 和 Vibrio artabrorum sp. nov. 这两个名称，其中 Vb 11.11(T)（ = CECT 7223(T) = LMG 24300(T)）和 Vb 11.8(T)（ = CECT 7226(T) = LMG 23865(T)）分别作为模式株。
