Departamento de Microbiología y Parasitología, CIBUS, Universidad de Santiago de Compostela, Campus Sur s/n, 15782 Santiago de Compostela, Spain.
Syst Appl Microbiol. 2013 Mar;36(2):96-100. doi: 10.1016/j.syapm.2012.11.005. Epub 2012 Dec 30.
Four motile facultative anaerobic marine isolates (Vb 10.8(T) [CECT 7225(T), CAIM 1869(T)], CMJ 9.4 [CECT 8091, CAIM 1870], CMJ 9.11 and Cmf 13.9), were obtained from cultured clams (Venerupis philippinarum and Venerupis decussata) in Galicia (NW Spain). These isolates were studied by a polyphasic approach, including a phylogenetic analysis based on sequences of 16S rRNA and five housekeeping genes atpA, recA, pyrH, rpoA and rpoD, that supported their inclusion in the Splendidus clade of the genus Vibrio, forming a well-defined group separated from the others species of the clade. DNA-DNA hybridizations with the type strains of species showing more than 98.5% 16S rRNA gene sequence similarity rendered values of hybridization below 60%. These isolates could be differentiated from the closest relatives on the basis of several phenotypic and chemotaxonomic features. These results demonstr8ated that the strains constitute a novel specie of the genus for which the name Vibrio toranzoniae sp. nov. is proposed, with Vb 10.8(T) (=CECT 7225(T)CAIM 1869(T)) as the type strain.
从加利西亚（西班牙西北部）养殖的蛤（菲律宾蛤仔和文蛤）中分离到四个可运动兼性厌氧海洋分离株（Vb 10.8(T)[CECT 7225(T)，CAIM 1869(T)]，CMJ 9.4[CECT 8091，CAIM 1870]，CMJ 9.11 和 Cmf 13.9）。这些分离株通过多相分析进行了研究，包括基于 16S rRNA 和五个看家基因 atpA、recA、pyrH、rpoA 和 rpoD 序列的系统发育分析，支持它们被归入弧菌属的 Splendidus 分支，形成一个与该分支的其他物种明显分离的明确群体。与具有超过 98.5% 16S rRNA 基因序列相似性的模式菌株的 DNA-DNA 杂交产生的杂交值低于 60%。这些分离株可以根据几种表型和化学分类特征与最接近的亲缘关系区分开来。这些结果表明，这些菌株构成了一个新的属种，建议将其命名为 Vibrio toranzoniae sp. nov.，Vb 10.8(T)=CECT 7225(T)=CAIM 1869(T)作为模式株。
