Mallinson G, Anstee D J, Avent N D, Ridgwell K, Tanner M J, Daniels G L, Tippett P, von dem Borne A E
Blood Group Reference Laboratory, South Western Regional Blood Transfusion Centre, Southmead, Bristol, UK.
Transfusion. 1990 Mar-Apr;30(3):222-5. doi: 10.1046/j.1537-2995.1990.30390194341.x.
The human red cell membrane components reacting with monoclonal antibody MB-2D10 were examined by immunoblotting. The antibody bound to a diffusely staining band extending from Mr 30,000 up to the high-molecular-weight region of the gel in normal membranes and in Rhnull U + membranes, but not in Rhnull U - membranes. Treatment of normal red cells with an endoglycosidase F-containing preparation destroyed the epitope recognized by MB-2D10. The reactivity of the antibody with purified preparations of Rh-related glycoproteins D30 polypeptide, D50 polypeptide, R6A32 polypeptide, and R6A45 polypeptide was also examined. Only the purified R6A45 and D50 components reacted with MB-2D10. These results show that MB-2D10 recognizes a carbohydrate-dependent epitope on the R6A45 and D50 group of Rh-related polypeptides. The results also suggest the possibility that the U antigen arises from interaction between glycophorin B and the Rh-related components D50 and R6A45.
通过免疫印迹法检测了与单克隆抗体MB - 2D10发生反应的人红细胞膜成分。在正常膜和Rhnull U +膜中，该抗体与一条弥散染色带结合，该带从分子量30,000延伸至凝胶的高分子量区域，但在Rhnull U -膜中则不结合。用含内切糖苷酶F的制剂处理正常红细胞会破坏MB - 2D10识别的表位。还检测了该抗体与Rh相关糖蛋白D30多肽、D50多肽、R6A32多肽和R6A45多肽纯化制剂的反应性。只有纯化的R6A45和D50成分与MB - 2D10发生反应。这些结果表明，MB - 2D10识别Rh相关多肽的R6A45和D50组上的一个碳水化合物依赖性表位。结果还提示了U抗原可能源于血型糖蛋白B与Rh相关成分D50和R6A45之间相互作用的可能性。
