Gardner B, Anstee D J, Mawby W J, Tanner M J, von dem Borne A E
Department of Haematology, St Mary's Hospital, Paddington, London, U.K.
Transfus Med. 1991 Jun;1(2):77-85. doi: 10.1111/j.1365-3148.1991.tb00013.x.
Twelve murine monoclonal antibodies, which react with human red cells of common Rh phenotype but give weak or negative reactions with Rh null erythrocytes, were used in quantitative binding assays and competitive binding assays to investigate the abundance and organization of polypeptides involved in the expression of antigens of the Rh blood group system. Antibodies of the R6A-type (R6A, BRIC-69, BRIC-207) and the 2D10-type (MB-2D10, LA18.18, LA23.40) recognize related structures and 100,000-200,000 molecules of each antibody bind maximally to erythrocytes of common Rh phenotype. Antibodies of the BRIC-125 type (BRICs 32, 122, 125, 126, 168, 211) recognize structures that are unrelated to those recognized by R6A-type and 2D10-type antibodies and between 10,000 and 50,000 antibody molecules bind maximally to erythrocytes of the common Rh phenotype. The binding of antibodies of the R6A-type and the 2D10-type, but not of antibodies of the BRIC-125-type could be partially inhibited by human anti-D antibodies (polyclonal and monoclonal) and a murine anti-e-like antibody. These results are consistent with evidence (Moore & Green 1987; Avent et al., 1988b) that the Rh blood group antigens are associated with a complex that comprises two groups of related polypeptides of M(r) 30,000 and M(r) 35,000-100,000, respectively, and suggest that there are 1-2 x 10(5) copies of this complex per erythrocyte. The polypeptide recognized by antibodies of the BRIC-125 type is likely to be associated with this complex.
12种鼠单克隆抗体与常见Rh血型表型的人类红细胞发生反应，但与Rh缺失红细胞的反应较弱或呈阴性，将其用于定量结合试验和竞争性结合试验，以研究参与Rh血型系统抗原表达的多肽的丰度和组织情况。R6A类型（R6A、BRIC-69、BRIC-207）和2D10类型（MB-2D10、LA18.18、LA23.40）的抗体识别相关结构，每种抗体100,000 - 200,000个分子与常见Rh血型表型的红细胞最大程度结合。BRIC-125类型（BRICs 32、122、125、126、168、211）的抗体识别的结构与R6A类型和2D10类型抗体识别的结构无关，10,000至50,000个抗体分子与常见Rh血型表型的红细胞最大程度结合。R6A类型和2D10类型抗体的结合，但不是BRIC-125类型抗体的结合，可被人抗-D抗体（多克隆和单克隆）和一种鼠抗-e样抗体部分抑制。这些结果与以下证据一致（Moore & Green，1987；Avent等人，1988b），即Rh血型抗原与一种复合物相关，该复合物分别包含两组相关多肽，分子量分别为30,000和35,000 - 100,000，并表明每个红细胞有1 - 2×10⁵个这种复合物拷贝数。BRIC-125类型抗体识别的多肽可能与该复合物相关。
