肺炎球菌肽聚糖-多糖调节小鼠中耳上皮细胞中的 Toll 样受体 2。
Pneumococcal peptidoglycan-polysaccharides regulate Toll-like receptor 2 in the mouse middle ear epithelial cells.
机构信息
Department of Otolaryngology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
出版信息
Pediatr Res. 2011 Feb;69(2):101-5. doi: 10.1203/PDR.0b013e3182055237.
Abstract
Toll-like receptor 2 (TLR2) plays a key role in the host defense against Gram staining positive (Gram) bacteria and their cell wall envelope components. However, little is known about the expression of TLR2 in the middle ear under otitis media (OM) conditions, and its role in the persistent otitis media with effusion (OME). In this study, we demonstrated that the pneumococcal cell wall component, peptidoglycan-polysaccharides (PGPS), activated the expression of TLR2 in the middle ear epithelial cells through the nuclear factor kappa B (NF-κB)-cytokine signaling pathway while I kappa B alpha mutant (IκBαM), a dominant negative inhibitor of NF-κB, abrogated the expression of TLR2 induced by PGPS. This study suggests that the existence of residual PGPS may maintain a low profile of cytokine production in the middle ear mucosa and thus contribute to the pathogenesis of OME.
摘要
Toll-like receptor 2 (TLR2) 在宿主防御革兰氏染色阳性（革兰氏）细菌及其细胞壁包膜成分中发挥关键作用。然而，关于 TLR2 在中耳炎（OM）条件下中耳中的表达及其在持续性分泌性中耳炎（OME）中的作用知之甚少。在这项研究中，我们表明肺炎球菌细胞壁成分肽聚糖-多糖（PGPS）通过核因子 kappa B（NF-κB）-细胞因子信号通路激活中耳上皮细胞中 TLR2 的表达，而 I kappa B alpha 突变体（IκBαM），NF-κB 的显性负抑制剂，可阻断 PGPS 诱导的 TLR2 表达。本研究表明，残留的 PGPS 的存在可能使中耳黏膜中的细胞因子产生保持低水平，从而有助于 OME 的发病机制。
相似文献
1
Pneumococcal peptidoglycan-polysaccharides regulate Toll-like receptor 2 in the mouse middle ear epithelial cells.肺炎球菌肽聚糖-多糖调节小鼠中耳上皮细胞中的 Toll 样受体 2。
Pediatr Res. 2011 Feb;69(2):101-5. doi: 10.1203/PDR.0b013e3182055237.
2
[Toll-like receptor 2 and Toll-like receptor 4 participates in mediation of acute otitis media and mortality in pneumococcal infections in mice].[Toll样受体2和Toll样受体4参与介导小鼠肺炎球菌感染中的急性中耳炎和死亡率]
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Dec;46(12):1009-18.
3
Peptidoglycan enhances proinflammatory cytokine expression through the TLR2 receptor, MyD88, phosphatidylinositol 3-kinase/AKT and NF-kappaB pathways in BV-2 microglia.肽聚糖通过 TLR2 受体、MyD88、磷脂酰肌醇 3-激酶/AKT 和 NF-κB 途径增强 BV-2 小胶质细胞中的促炎细胞因子表达。
Int Immunopharmacol. 2010 Aug;10(8):883-91. doi: 10.1016/j.intimp.2010.04.026. Epub 2010 May 5.
4
The tumor suppressor cylindromatosis (CYLD) acts as a negative regulator for toll-like receptor 2 signaling via negative cross-talk with TRAF6 AND TRAF7.肿瘤抑制因子圆柱瘤蛋白（CYLD）通过与肿瘤坏死因子受体相关因子6（TRAF6）和肿瘤坏死因子受体相关因子7（TRAF7）的负向相互作用，作为Toll样受体2信号通路的负调节因子。
J Biol Chem. 2005 Dec 9;280(49):41111-21. doi: 10.1074/jbc.M509526200. Epub 2005 Oct 17.
5
Otitis media induced by peptidoglycan-polysaccharide (PGPS) in TLR2-deficient (Tlr2(-/-)) mice for developing drug therapy.在缺乏Toll样受体2（Tlr2(-/-)）的小鼠中，由肽聚糖-多糖（PGPS）诱导的中耳炎用于开发药物治疗。
Infect Genet Evol. 2015 Oct;35:194-203. doi: 10.1016/j.meegid.2015.08.019. Epub 2015 Aug 18.
6
Role for Toll-like receptor 2 in the immune response to Streptococcus pneumoniae infection in mouse otitis media.Toll样受体2在小鼠中耳炎肺炎链球菌感染免疫反应中的作用。
Infect Immun. 2009 Jul;77(7):3100-8. doi: 10.1128/IAI.00204-09. Epub 2009 May 4.
7
Association of microRNA 146 with middle ear hyperplasia in pediatric otitis media.微小RNA 146与小儿中耳炎中耳增生的关联。
Int J Pediatr Otorhinolaryngol. 2016 Sep;88:104-8. doi: 10.1016/j.ijporl.2016.06.056. Epub 2016 Jul 2.
8
Pneumococcal peptidoglycan-polysaccharides induce the expression of interleukin-8 in airway epithelial cells by way of nuclear factor-kappaB, nuclear factor interleukin-6, or activation protein-1 dependent mechanisms.肺炎球菌肽聚糖-多糖通过核因子κB、核因子白细胞介素-6或活化蛋白-1依赖性机制诱导气道上皮细胞中白细胞介素-8的表达。
Laryngoscope. 2007 Jan;117(1):86-91. doi: 10.1097/01.mlg.0000244182.81768.31.
9
Peptidoglycan induces bradykinin receptor 1 expression through Toll-like receptor 2 and NF-κB signaling pathway in human nasal mucosa-derived fibroblasts of chronic rhinosinusitis patients.肽聚糖通过 Toll 样受体 2 和 NF-κB 信号通路诱导慢性鼻-鼻窦炎患者鼻黏膜成纤维细胞中缓激肽受体 1 的表达。
J Cell Physiol. 2018 Sep;233(9):7226-7238. doi: 10.1002/jcp.26553. Epub 2018 Mar 25.
10
TBX21 participates in innate immune response by regulating Toll-like receptor 2 expression in Streptococcus pneumoniae infections.TBX21 通过调节肺炎链球菌感染中的 Toll 样受体 2 表达参与固有免疫反应。
Mol Oral Microbiol. 2014 Oct;29(5):233-43. doi: 10.1111/omi.12061. Epub 2014 Jul 15.
引用本文的文献
1
Autophagy Contributes to the Rapamycin-Induced Improvement of Otitis Media.自噬有助于雷帕霉素诱导的中耳炎改善。
Front Cell Neurosci. 2022 Jan 28;15:753369. doi: 10.3389/fncel.2021.753369. eCollection 2021.
2
Innate Immunity in the Middle Ear Mucosa.中耳黏膜的固有免疫。
Front Cell Infect Microbiol. 2021 Oct 29;11:764772. doi: 10.3389/fcimb.2021.764772. eCollection 2021.
3
Dysregulation of immune response in otitis media.中耳炎中免疫反应的失调。
Expert Rev Mol Med. 2021 Aug 18;23:e10. doi: 10.1017/erm.2021.10.
4
Toll-Like Receptors: Expression and Roles in Otitis Media.Toll 样受体：中耳炎中的表达和作用。
Int J Mol Sci. 2021 Jul 23;22(15):7868. doi: 10.3390/ijms22157868.
5
Suppression of lncRNA MALAT1 Reduces LPS- or IL-17A-Induced Inflammatory Response in Human Middle Ear Epithelial Cells via the NF-B Signaling Pathway.长链非编码 RNA MALAT1 的抑制通过 NF-κB 信号通路减轻 LPS 或 IL-17A 诱导的人中耳上皮细胞的炎症反应。
Biomed Res Int. 2021 Jan 6;2021:8844119. doi: 10.1155/2021/8844119. eCollection 2021.
6
Role of Endoplasmic Reticulum Stress in Otitis Media.内质网应激在中耳炎中的作用。
Front Genet. 2020 May 27;11:495. doi: 10.3389/fgene.2020.00495. eCollection 2020.
7
Mathematical Modeling of Colonization, Invasive Infection and Treatment.定植、侵袭性感染及治疗的数学建模
Front Physiol. 2017 Mar 2;8:115. doi: 10.3389/fphys.2017.00115. eCollection 2017.
8
Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils.维生素 D 促进肺炎球菌杀伤并调节原代人中性粒细胞的炎症反应。
J Innate Immun. 2017;9(4):375-386. doi: 10.1159/000455969. Epub 2017 Feb 28.
9
TLR2 promotes macrophage recruitment and Streptococcus pneumoniae clearance during mouse otitis media.Toll样受体2（TLR2）在小鼠中耳炎期间促进巨噬细胞募集和肺炎链球菌清除。
Pediatr Res. 2016 Dec;80(6):886-893. doi: 10.1038/pr.2016.154. Epub 2016 Jul 27.
10
Otitis media induced by peptidoglycan-polysaccharide (PGPS) in TLR2-deficient (Tlr2(-/-)) mice for developing drug therapy.在缺乏Toll样受体2（Tlr2(-/-)）的小鼠中，由肽聚糖-多糖（PGPS）诱导的中耳炎用于开发药物治疗。
Infect Genet Evol. 2015 Oct;35:194-203. doi: 10.1016/j.meegid.2015.08.019. Epub 2015 Aug 18.
本文引用的文献
1
Sonic hedgehog (SHH) promotes the differentiation of mouse cochlear neural progenitors via the Math1-Brn3.1 signaling pathway in vitro. Sonic hedgehog (SHH) 通过 Math1-Brn3.1 信号通路促进体外培养的小鼠耳蜗神经前体细胞的分化。
J Neurosci Res. 2010 Apr;88(5):927-35. doi: 10.1002/jnr.22286.
2
Role for Toll-like receptor 2 in the immune response to Streptococcus pneumoniae infection in mouse otitis media.Toll样受体2在小鼠中耳炎肺炎链球菌感染免疫反应中的作用。
Infect Immun. 2009 Jul;77(7):3100-8. doi: 10.1128/IAI.00204-09. Epub 2009 May 4.
3
Id1 induces the proliferation of cochlear sensory epithelial cells via the nuclear factor-kappaB/cyclin D1 pathway in vitro.在体外，Id1通过核因子-κB/细胞周期蛋白D1途径诱导耳蜗感觉上皮细胞增殖。
J Neurosci Res. 2007 Feb 15;85(3):515-24. doi: 10.1002/jnr.21133.
4
Pneumococcal peptidoglycan-polysaccharides induce the expression of interleukin-8 in airway epithelial cells by way of nuclear factor-kappaB, nuclear factor interleukin-6, or activation protein-1 dependent mechanisms.肺炎球菌肽聚糖-多糖通过核因子κB、核因子白细胞介素-6或活化蛋白-1依赖性机制诱导气道上皮细胞中白细胞介素-8的表达。
Laryngoscope. 2007 Jan;117(1):86-91. doi: 10.1097/01.mlg.0000244182.81768.31.
5
Characterization of a temperature-sensitive mouse middle ear epithelial cell line.一种温度敏感型小鼠中耳上皮细胞系的特性分析。
Acta Otolaryngol. 2005 Aug;125(8):823-9. doi: 10.1080/00016480510031533.
6
Analysis by cDNA microarrays of altered gene expression in middle ears of rats following pneumococcal infection.肺炎球菌感染后大鼠中耳基因表达改变的cDNA微阵列分析。
Int J Pediatr Otorhinolaryngol. 2002 Sep 24;65(3):203-11. doi: 10.1016/s0165-5876(02)00130-1.
7
Toll-like receptors: critical proteins linking innate and acquired immunity.Toll样受体：连接先天免疫和后天免疫的关键蛋白。
Nat Immunol. 2001 Aug;2(8):675-80. doi: 10.1038/90609.
8
NF-kappaB signals induce the expression of c-FLIP.核因子-κB信号诱导细胞凋亡抑制蛋白长链的表达。
Mol Cell Biol. 2001 Aug;21(16):5299-305. doi: 10.1128/MCB.21.16.5299-5305.2001.
9
Activation of NF-kappa B by nontypeable Hemophilus influenzae is mediated by toll-like receptor 2-TAK1-dependent NIK-IKK alpha /beta-I kappa B alpha and MKK3/6-p38 MAP kinase signaling pathways in epithelial cells.不可分型流感嗜血杆菌激活核因子-κB是通过上皮细胞中Toll样受体2-TAK1依赖的NIK-IKKα/β-IκBα以及MKK3/6-p38丝裂原活化蛋白激酶信号通路介导的。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8774-9. doi: 10.1073/pnas.151236098. Epub 2001 Jul 3.
10
Characterization of mucins in human middle ear and Eustachian tube.人中耳和咽鼓管中黏蛋白的特性分析
Am J Physiol Lung Cell Mol Physiol. 2001 Jun;280(6):L1157-67. doi: 10.1152/ajplung.2001.280.6.L1157.
Zotero 插件