Shiryaev Sergey A, Strongin Alex Y
Inflammatory & Infectious Disease Center, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA.
Future Virol. 2010 Sep 1;5(5):593-606. doi: 10.2217/fvl.10.39.
Flaviviruses have a single-strand, positive-polarity RNA genome that encodes a single polyprotein. The polyprotein is comprised of seven nonstructural (NS) and three structural proteins. The N- and C-terminal parts of NS3 represent the serine protease and the RNA helicase, respectively. The cleavage of the polyprotein by the protease is required to produce the individual viral proteins, which assemble a new viral progeny. Conversely, inactivation of the protease blocks viral infection. Both the protease and the helicase are conserved among flaviviruses. As a result, NS3 is a promising drug target in flaviviral infections. This article examines the West Nile virus NS3 with an emphasis on the structural and functional parameters of the protease, the helicase and their cofactors.
黄病毒具有单链、正链RNA基因组，该基因组编码一种单一的多聚蛋白。多聚蛋白由七种非结构（NS）蛋白和三种结构蛋白组成。NS3的N端和C端部分分别代表丝氨酸蛋白酶和RNA解旋酶。蛋白酶对多聚蛋白的切割是产生单个病毒蛋白所必需的，这些病毒蛋白组装成新的病毒后代。相反，蛋白酶的失活会阻断病毒感染。蛋白酶和解旋酶在黄病毒中都是保守的。因此，NS3是黄病毒感染中一个有前景的药物靶点。本文研究西尼罗河病毒NS3，重点关注蛋白酶、解旋酶及其辅助因子的结构和功能参数。
