Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 6º andar/sala645, Porto Alegre, RS, 90035-003, Brazil.
Inflamm Res. 2011 Apr;60(4):347-56. doi: 10.1007/s00011-010-0277-2. Epub 2010 Nov 13.
The role of NO in muscle injury is not clear.
We examined the involvement of the NO system in the development of muscle damage in an experimental model of crush injury. The animals were divided into four groups: (1) control (CO), (2) sham trauma, (3) trauma, (4) trauma + L -NAME, in two experimental phases, 24 h and 7 days after injury.
Twenty-four hours post-trauma, the crushed muscle was characterized by an intense inflammatory reaction. These changes were accompanied by increased oxidative damage, increased cytokine mRNA transcription, NF-κB binding ability and TGF-β growth factor expression in the gastrocnemius muscle. Treatment with L: -NAME markedly decreased these histological and molecular abnormalities at 24 h. However, at 7 days post-trauma, increased collagen formation was observed in the L: -NAME group.
These findings indicate that NO is involved in the balance between fibrosis and healing with regeneration.
NO 在肌肉损伤中的作用尚不清楚。
我们研究了 NO 系统在挤压伤实验模型中肌肉损伤发展中的作用。动物分为四组：(1)对照组（CO）、(2)假手术组、(3)创伤组、(4)创伤+L-NAME 组，在损伤后 24 小时和 7 天进行两个实验阶段。
创伤后 24 小时，挤压肌肉表现出强烈的炎症反应。这些变化伴随着腓肠肌中氧化损伤增加、细胞因子 mRNA 转录增加、NF-κB 结合能力和 TGF-β生长因子表达增加。在 24 小时时，用 L-NAME 治疗可显著减少这些组织学和分子异常。然而，在创伤后 7 天，L-NAME 组观察到胶原形成增加。
这些发现表明，NO 参与了纤维化和再生性愈合之间的平衡。
