Jung Mun Yhung, Choi Nak Jin, Oh Chan Ho, Shin Hyun Kyung, Yoon Suk Hoo
College of Food Science, Woosuk University, Samyre-Eup, Jeollabuk-Do, Republic of Korea.
Lipids. 2011 Mar;46(3):287-95. doi: 10.1007/s11745-010-3495-z. Epub 2010 Nov 13.
Prostate cancer is the second leading cause of male deaths due to cancer in the United States. Hydrogenated vegetable oils have been suspected of inducing adverse health effects, including atherosclerosis and cancer. Here we report that a selectively hydrogenated soybean oil (SHSO) containing a high quantity of conjugated linoleic acids showed remarkably strong anticarcinogenic activity against prostate cancer in the rat model (Copenhagen rats with MAT-LyLu syngeneic rat prostate cancer cells) study in vivo and human prostate carcinoma cell lines studies in vitro, as compared with native soybean oil. A 5% dietary supplementation with SHSO inhibited the growth of prostate cancer by 80% in vivo. The TUNEL method and immunohistochemical staining assays of bax, bcl-2, and survivin clearly showed that SHSO induced prostate cancer cell apoptosis in the tested rats. DNA fragmentation analysis in vitro further confirmed the apoptotic activity of SHSO on the MAT-LyLu prostate cancer cells. The SHSO also showed strong cytotoxicity on human prostate cancer cells (DU145 and PC3). This represents the first report demonstrating the significant anticancer activities of hydrogenated vegetable oils at low levels of dietary supplementation.
前列腺癌是美国男性因癌症死亡的第二大主要原因。氢化植物油一直被怀疑会引发不良健康影响，包括动脉粥样硬化和癌症。在此我们报告，与天然大豆油相比，一种含有大量共轭亚油酸的选择性氢化大豆油（SHSO）在体内大鼠模型（携带MAT-LyLu同基因大鼠前列腺癌细胞的哥本哈根大鼠）研究以及体外人前列腺癌细胞系研究中，对前列腺癌显示出显著强大的抗癌活性。在饮食中添加5%的SHSO可在体内抑制80%的前列腺癌生长。TUNEL法以及对bax、bcl-2和survivin的免疫组化染色分析清楚地表明，SHSO在受试大鼠中诱导前列腺癌细胞凋亡。体外DNA片段化分析进一步证实了SHSO对MAT-LyLu前列腺癌细胞的凋亡活性。SHSO对人前列腺癌细胞（DU145和PC3）也显示出强大的细胞毒性。这是第一份证明在低水平饮食补充时氢化植物油具有显著抗癌活性的报告。
