Department of Materials Science and Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
J Microsc. 2010 Dec;240(3):173-80. doi: 10.1111/j.1365-2818.2010.03399.x. Epub 2010 Aug 26.
Laser scanning confocal microscopes are essential and ubiquitous tools in the biological, biochemical and biomedical sciences, and play a similar role to scanning electron microscopes in materials science. However, modern laser scanning confocal microscopes have a number of advantages for the study of materials, in addition to their obvious uses for high resolution reflected and transmitted light optical microscopy. In this paper, we provide several examples that exploit the laser scanning confocal microscope's capabilities of pseudo-infinite depth of field imaging, topographic imaging, photo-stimulated luminescence imaging and Raman spectroscopic imaging.
激光扫描共焦显微镜是生物、生化和生物医学科学中不可或缺的通用工具,其在材料科学中的作用类似于扫描电子显微镜。然而,除了用于高分辨率反射和透射光显微镜之外,现代激光扫描共焦显微镜在材料研究方面具有许多优势。在本文中,我们提供了一些利用激光扫描共焦显微镜的近似无限景深成像、形貌成像、光致发光成像和拉曼光谱成像功能的示例。