Department of Nutritional Toxicology, Institute of Nutrition, Friedrich Schiller University Jena, Jena, Germany.
Free Radic Biol Med. 2011 Jan 15;50(2):305-12. doi: 10.1016/j.freeradbiomed.2010.11.012. Epub 2010 Nov 13.
Photodynamic therapy (PDT) is a potential tool in cancer treatment. Today this therapy is established among others for the treatment of nonmelanoma skin cancer. However, the more dangerous skin cancer--the melanoma--still has to be removed by surgery. Therefore, we investigated the effects of PDT and additional administration of heme oxygenase I (HO-I) and poly(ADP-ribose) polymerase (PARP) inhibitors on the treatment of melanoma cells in comparison to nonmalignant keratinocytes. Therefore, cocultures were established with WM451LU melanoma cells and HaCaT keratinocytes. In the coculture some 65% melanoma cells and 35% HaCaT cells were present before PDT, whereas after PDT the proportion was 41% melanoma cells and 59% HaCaT cells. Combination of both inhibitors improves these results to only 16% melanoma cells and 84% HaCaT cells. PDT is, therefore, a potent skin cancer treatment, which might also be interesting for melanoma treatment. The cytotoxic effects of PDT are largely mediated by ROS. Addition of HO-I and PARP inhibitors could improve the efficiency of photodynamic treatment.
光动力疗法 (PDT) 是癌症治疗的一种有潜力的手段。目前，这种疗法已被广泛应用于非黑色素瘤皮肤癌的治疗。然而，更危险的皮肤癌——黑色素瘤——仍需通过手术切除。因此，我们研究了 PDT 以及血红素加氧酶 I (HO-I) 和聚 ADP-核糖聚合酶 (PARP) 抑制剂的联合应用对黑色素瘤细胞治疗的效果，同时将其与非恶性角质形成细胞进行了比较。为此，我们建立了 WM451LU 黑色素瘤细胞和 HaCaT 角质形成细胞的共培养物。在 PDT 之前，共培养物中约有 65%的黑色素瘤细胞和 35%的 HaCaT 细胞，而 PDT 后，黑色素瘤细胞和 HaCaT 细胞的比例分别为 41%和 59%。两种抑制剂的联合使用将这些结果改善至仅 16%的黑色素瘤细胞和 84%的 HaCaT 细胞。因此，PDT 是一种有效的皮肤癌治疗方法，对于黑色素瘤的治疗也可能具有重要意义。PDT 的细胞毒性作用主要由 ROS 介导。添加 HO-I 和 PARP 抑制剂可以提高光动力治疗的效率。
