Hozumi Akira, Osaki Makoto, Sakamoto Kazutaka, Goto Hisataka, Fukushima Tatsuya, Baba Hideo, Shindo Hiroyuki
Departement of Orthopaedic Surgery, Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan.
Biomed Res. 2010 Oct;31(5):281-6. doi: 10.2220/biomedres.31.281.
Several studies have demonstrated the association of plasminogen activator inhibitor-1 (PAI-1) with osteonecrosis, but the underlying mechanism of osteonecrosis and its relationship with local PAI-1 is not clear. The objective of this study was to evaluate PAI-1 production by primary human bone marrow adipocytes and the effects of glucocorticoid administration. Bone marrow was obtained from 25 individuals during prosthetic insertion. Mature adipocytes were cultured for 24 h with or without dexamethasone. PAI-1, adiponectin, tumor necrosing factor-α (TNFα) expression were measured by latex photometric immunoassay or RT-PCR. Adiponectin, TNFα and PAI-1 were detected in all culture media. PAI-1 expression was significantly increased by treatment with 10(-6) mol/L dexamethasone up to 24 h in protein and mRNA levels, while the levels of other adipokines did not change by dexamethasone. These results suggest that bone marrow adipocytes may play important roles for the development of glucocorticoid-induced osteonecrotic diseases by enhancing PAI-1 expression.
多项研究已证实纤溶酶原激活物抑制剂-1（PAI-1）与骨坏死有关，但骨坏死的潜在机制及其与局部PAI-1的关系尚不清楚。本研究的目的是评估原代人骨髓脂肪细胞中PAI-1的产生以及糖皮质激素给药的影响。在假体植入期间从25名个体获取骨髓。成熟脂肪细胞在有或没有地塞米松的情况下培养24小时。通过乳胶比浊免疫测定法或RT-PCR测量PAI-1、脂联素、肿瘤坏死因子-α（TNFα）的表达。在所有培养基中均检测到脂联素、TNFα和PAI-1。在蛋白质和mRNA水平上，用10（-6）mol/L地塞米松处理长达24小时可使PAI-1表达显著增加，而其他脂肪因子的水平不受地塞米松影响。这些结果表明，骨髓脂肪细胞可能通过增强PAI-1表达在糖皮质激素诱导的骨坏死性疾病的发展中起重要作用。
