Department of Biophysics, Biology Faculty of the Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia.
Curr Microbiol. 2011 Mar;62(3):962-7. doi: 10.1007/s00284-010-9811-2. Epub 2010 Nov 16.
Antibacterial effects of the electromagnetic irradiation (EMI) of 51.8 and 53 GHz frequencies with low intensity (the flux capacity of 0.06 mW/cm(2)) and non-thermal action were investigated upon direct irradiation of E. coli K12. Significant decrease in bacterial growth rate and in the number of viable cells, marked change in H(+) and K(+) transport across membrane were shown. Subsequent addition of kanamycin or ceftriaxone (15 or 0.4 μM, respectively) enhanced the effects of irradiation. This was maximally achieved at the frequency of 53 GHz. These all might reveal membrane as probable target for antibacterial effects. Apparently, the action of EMI on bacteria might lead to changed membrane properties and to antibiotic resistance. The results should improve using extremely high frequency EMI in combination with antibiotics in biotechnology, therapeutic practice, and food industry.
研究了低强度（通量密度为 0.06 mW/cm(2)）和非热作用的 51.8 和 53GHz 频率的电磁辐射（EMI）对大肠杆菌 K12 的直接照射的抗菌作用。结果表明，细菌的生长速度和存活细胞数量显著下降，膜 H(+)和 K(+)转运明显改变。随后加入卡那霉素或头孢曲松（分别为 15 或 0.4μM）增强了照射的效果。在 53GHz 时达到最大值。这一切都可能表明膜是抗菌作用的可能靶点。显然，EMI 对细菌的作用可能导致膜性质的改变和抗生素耐药性的产生。这些结果可能会提高在生物技术、治疗实践和食品工业中结合使用超高频率 EMI 和抗生素的效果。
