State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Mailbox 365, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Mol Biol Rep. 2011 Apr;38(4):2649-56. doi: 10.1007/s11033-010-0406-6. Epub 2010 Nov 16.
Cell-penetrating peptides can carry a variety of biologically active molecules into cells. Here we have identified a novel CPP derived from the C-terminus of human extracellular superoxide dismutase (hC-SOD3) which was shown to be located throughout in the cytoplasm and nucleus by fluorescence microscopy investigation. Furthermore, when apoptin fused to hC-SOD3, it was translocated efficiently into HeLa cells resulting in antitumor activities. This study shows that hC-SOD3 has the potential to penetrate and translocate cargo molecules into cells and has no cytotoxicity at effective concentration.
细胞穿透肽可以将各种生物活性分子带入细胞。在这里，我们鉴定了一种新型 CPP，它来源于人细胞外超氧化物歧化酶 (hC-SOD3) 的 C 端，荧光显微镜研究表明该 CPP 位于细胞质和细胞核中。此外，当凋亡素与 hC-SOD3 融合时，它能有效地转位进入 HeLa 细胞，从而发挥抗肿瘤活性。本研究表明，hC-SOD3 具有穿透和转位细胞内货物分子的潜力，在有效浓度下无细胞毒性。
