Voncken Jan Willem
Department of Molecular Genetics, Maastricht University Medical Center, Maastricht, The Netherlands.
Methods Mol Biol. 2011;693:11-36. doi: 10.1007/978-1-60761-974-1_2.
Introduction of germ line mutations in mice via genetic engineering involves alterations of the structure and characteristics of genes. These alterations are mostly introduced via molecular genetic technology either in embryonal stem cells or in one-cell stage embryos. This chapter describes classic biotechnological methods used to generate mice from modified pre-implantation embryos.
