转基因设计。

van de Sluis Bart, Voncken Jan Willem

Department of Pathology & Medical Biology, Medical Biology Section, Molecular Genetics, University Medical Center Groningen, University of Gronignen, Groningen, The Netherlands.

Methods Mol Biol. 2011;693:89-101. doi: 10.1007/978-1-60761-974-1_6.

Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.

转基因动物是用于了解基因生物学功能的强大小鼠模型。本章简要概述了设计转基因时的要求和注意事项。此外，还讨论了为成功设计和培育转基因小鼠模型而必须预先做出的潜在重要选择。本章还提供了用于显微注射的DNA纯化方法。