MOE Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou 310058, China.
J Chromatogr A. 2010 Dec 24;1217(52):8205-11. doi: 10.1016/j.chroma.2010.10.097. Epub 2010 Oct 30.
A new molecularly imprinted polymer (MIP) targeting to quinolones (Qs) and tetracyclines (TCs) was synthesized using itaconic acid (ITA) and ciprofloxacin (CIP) as a functional monomer and template molecule, respectively. Factors affecting the overall performance of MIP were investigated, and the results showed that Fe(3+) ion play a vital role in the formation of MIP with high molecular imprinting effect. Meanwhile, the chelating ability of monomer, species of template molecule, as well as the molar ratio of monomer and template also contribute to the performance of the obtained MIP. Cyclic voltammetry verified that, with the participation of Fe(3+) ions, a ternary complex of ITA-Fe(3+)-CIP could be formed before polymerization. Compared with conventional MIP prepared from commonly used monomer, methacrylic acid (MAA), the new MIP show significantly enhanced molecular imprinting effect and higher capacity for specific adsorption of target compounds as revealed by static and dynamic binding experiments. The MIP was successfully used as solid-phase extraction (SPE) adsorbent for enriching a broad spectrum of antibiotics containing beta-diketone structure from surface water sample. HPLC detection showed that high recovery rate (78.6-113.6%) was found in these spiked antibiotics, whereas recovery rate for the non structurally related drugs, epinephrine (EP) and dopamine (DOPA), was very low (4.7-7.6%) on the MIP cartridges. The results demonstrate that the MIP prepared by the strategy proposed in this work, could specifically target to a series of structurally related antibiotics containing beta-diketone structure.
一种新型的喹诺酮类（Qs）和四环素类（TCs）分子印迹聚合物（MIP）采用衣康酸（ITA）和环丙沙星（CIP）分别作为功能单体和模板分子合成。研究了影响 MIP 整体性能的因素，结果表明Fe(3+)离子在形成具有高分子印迹效应的 MIP 中起着至关重要的作用。同时，单体的螯合能力、模板分子的种类以及单体和模板的摩尔比也有助于获得 MIP 的性能。循环伏安法验证了在聚合前，在 Fe(3+)离子的参与下，ITA-Fe(3+)-CIP 可以形成三元配合物。与通常使用的单体甲基丙烯酸（MAA）制备的常规 MIP 相比，新的 MIP 显示出明显增强的分子印迹效应和更高的目标化合物特异性吸附能力，这通过静态和动态结合实验得到证实。该 MIP 成功用作固相萃取（SPE）吸附剂，用于从地表水样品中富集具有β-二酮结构的广谱抗生素。HPLC 检测表明，在这些被添加的抗生素中，回收率很高（78.6-113.6%），而在 MIP 小柱上，非结构相关药物肾上腺素（EP）和多巴胺（DOPA）的回收率非常低（4.7-7.6%）。结果表明，通过本工作提出的策略制备的 MIP 可以特异性地针对一系列具有β-二酮结构的结构相关抗生素。
