Department of Physiology, Medical School, Institute for Medical Sciences, Chonbuk National University, JeonJu, 561-180, Republic of Korea.
Mol Biol Rep. 2011 Apr;38(4):2349-58. doi: 10.1007/s11033-010-0368-8. Epub 2010 Nov 17.
Propyl gallate (PG) as a synthetic antioxidant exerts a variety of effects on tissue and cell functions. Here, we investigated the effects of MAPK (MEK, JNK and p38) inhibitors on PG-treated HeLa cells in relation to cell death, ROS and GSH levels. PG induced cell growth inhibition and apoptosis in HeLa cells, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm). ROS levels were increased or decreased in PG-treated HeLa cells depending on the incubation times. PG also increased GSH depleted cell numbers in HeLa cells. All the MAPK inhibitors slightly enhanced cell growth inhibition, death and MMP (ΔΨm) loss, and increased ROS levels in PG-treated HeLa cells. However, MAPK inhibitors did not significantly affect GSH depletion in PG-treated cells. In conclusion, the enhanced effect of MAPK inhibitors on PG-induced HeLa cell death was accompanied by increasing ROS levels but the effect was not related to changes of GSH level.
没食子酸丙酯（PG）作为一种合成抗氧化剂，对组织和细胞功能有多种影响。在这里，我们研究了 MAPK（MEK、JNK 和 p38）抑制剂对与细胞死亡、ROS 和 GSH 水平相关的 PG 处理的 HeLa 细胞的影响。PG 诱导 HeLa 细胞生长抑制和凋亡，伴随着线粒体膜电位（MMP；ΔΨm）的丧失。PG 处理的 HeLa 细胞中的 ROS 水平随孵育时间的不同而增加或减少。PG 还增加了 GSH 耗竭的 HeLa 细胞数量。所有 MAPK 抑制剂都轻微增强了 PG 处理的 HeLa 细胞生长抑制、死亡和 MMP（ΔΨm）丧失，并增加了 ROS 水平。然而，MAPK 抑制剂对 PG 处理细胞中 GSH 耗竭的影响不显著。总之，MAPK 抑制剂对 PG 诱导的 HeLa 细胞死亡的增强作用伴随着 ROS 水平的增加，但与 GSH 水平的变化无关。
