Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Science, No. 9 East Shanshui Rd., Wuxi, China.
In Vitro Cell Dev Biol Anim. 2011 Jan;47(1):10-5. doi: 10.1007/s11626-010-9359-2. Epub 2010 Nov 17.
The present study aims to evaluate the hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract on the carbon tetrachloride (CCl(4))-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. H. sabdariffa extract (100, 200, and 400 μg/mL) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4). CCl(4) at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment and pre- and post-treatment of the hepatocytes with H. sabdariffa extract significantly reduced the elevated levels of LDH, GOT, GPT, and MDA and increased the reduced activities of SOD and GSH-Px in a dose-dependent manner; post-treatment did not show any protective effect. The results suggest that H. sabdariffa extract can be potentially used for preventing rather than curing liver diseases in fish.
本研究旨在评估玫瑰茄提取物对四氯化碳（CCl4）诱导的鱼类肝细胞损伤的保肝和抗氧化作用，并为其是否可作为水产养殖中肝脏疾病的潜在药物提供依据。在向鲤鱼原代肝细胞培养物中孵育 CCl4 之前（预处理）、之后（后处理）以及之前和之后（预处理和后处理）添加 100、200 和 400μg/mL 的玫瑰茄提取物。培养基中 8mM 的 CCl4 可显著提高乳酸脱氢酶（LDH）、谷氨酸草酰乙酸转氨酶（GOT）、谷氨酸丙酮酸转氨酶（GPT）和丙二醛（MDA）的水平，显著降低超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。用玫瑰茄提取物预处理和预处理及后处理肝细胞可显著降低 LDH、GOT、GPT 和 MDA 的升高水平，并呈剂量依赖性增加 SOD 和 GSH-Px 的活性；后处理则没有显示出任何保护作用。结果表明，玫瑰茄提取物可用于预防鱼类肝脏疾病，而非治疗。
