人脂肪来源干细胞的纯化与表征方法。

Methods for the purification and characterization of human adipose-derived stem cells.

作者信息

Gronthos Stan, Zannettino Andrew C W

机构信息

Mesenchymal Stem Cell Group, Department of Haematology, Institute of Medical and Veterinary Science/Hanson Institute and Centre for Stem Cell Research, University of Adelaide, Adelaide, SA, Australia.

出版信息

Methods Mol Biol. 2011;702:109-20. doi: 10.1007/978-1-61737-960-4_9.

DOI:10.1007/978-1-61737-960-4_9

PMID:21082398

Abstract

Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion. Recent studies have demonstrated that ASC are most likely derived from a perivascular niche within highly vascularised fat tissue, analogous to different mesenchymal cell populations identified in other tissues throughout the body. The following chapter describes techniques to prospectively isolate clonogenic ASC from adult human adipose tissue using antibodies directed against perivascular markers and methods to immunophenotypically characterize their ex vivo expanded progeny.

摘要

外周脂肪组织包含一群克隆性前体细胞，称为脂肪来源干细胞（ASC），这些细胞在体外扩增后仍保留分化为多种细胞类型的能力，包括成骨细胞、脂肪细胞、软骨细胞、肌细胞和神经细胞。最近的研究表明，ASC很可能来源于高度血管化脂肪组织中的血管周围微环境，类似于在全身其他组织中鉴定出的不同间充质细胞群体。以下章节描述了使用针对血管周围标志物的抗体从成人脂肪组织中前瞻性分离克隆性ASC的技术，以及对其体外扩增后代进行免疫表型特征分析的方法。