诱导脂肪干细胞软骨生成的三维培养系统。
Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.
作者信息
Estes Bradley T, Guilak Farshid
机构信息
Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Durham, NC, USA.
出版信息
Methods Mol Biol. 2011;702:201-17. doi: 10.1007/978-1-61737-960-4_15.
Abstract
Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.
摘要
干细胞可以很容易地从脂肪组织中大量获取，用于软骨修复或再生的组织工程方法。在本章中，我们描述了我们实验室用于脂肪来源干细胞（ASC）软骨生成诱导的体外组织工程模型。除了合适的生长因子环境外，软骨生成还要求细胞在三维（3D）培养中保持圆形形态，因此生物材料支架的特性在ASC分化中也起着关键作用。还介绍了评估软骨生成的组织学和免疫组织学方法。一般来说，在这些模型系统中评估ASC软骨生成需要10至12周。
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