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免疫化学中的电化学检测策略。

Strategies for electrochemical detection in immunochemistry.

作者信息

Centi Sonia, Laschi Serena, Mascini Marco

机构信息

Università degli Studi di Firenze, Dipartimento di Chimica, Polo Scientifico, via della Lastruccia 3, 50019 Sesto Fiorentino, Firenze, Italy.

出版信息

Bioanalysis. 2009 Oct;1(7):1271-91. doi: 10.4155/bio.09.106.

Abstract

In this review, the current status of research in electrochemical immunosensors is considered. We primarily focus on label-free and enzyme-labeled immunosensors, and the analytical capabilities of these devices are discussed. Moreover, the use of magnetic beads as new materials for immunosensors coupled with electrochemical sensing is also described, together with the application of new molecules such as aptamers as specific biorecognition elements. Examples of the applicability of these devices in solving various analytical problems in clinical, environmental and food fields are reported. Finally, the prospects for the further development of immunosensor technologies are shown.

摘要

在本综述中,我们考虑了电化学免疫传感器的研究现状。我们主要关注无标记和酶标记免疫传感器,并讨论了这些设备的分析能力。此外,还描述了将磁珠用作免疫传感器的新材料并结合电化学传感,以及适体等新分子作为特异性生物识别元件的应用。报告了这些设备在解决临床、环境和食品领域各种分析问题中的适用性实例。最后,展示了免疫传感器技术进一步发展的前景。

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