Jiang Xuesong, Li Dongyang, Xu Xia, Ying Yibin, Li Yanbin, Ye Zunzhong, Wang Jianping
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, People's Republic of China.
Biosens Bioelectron. 2008 Jun 15;23(11):1577-87. doi: 10.1016/j.bios.2008.01.035. Epub 2008 Feb 13.
Immunosensors are biosensors that use antibodies or antigens as the specific sensing element and provide concentration-dependent signals. There is great potential in the applications of immunosensing technologies for rapid detection of pesticide residues in food and environment. This paper presents an overview of various transduction systems, such as electrochemical, optical, piezoelectric, and nanomechanics methods, which have been reported in the literature in the design and fabrication of immunosensors for pesticide detection. Various immobilization protocols used for formation of a biorecognition interface are also discussed. In addition, techniques of regeneration, signal amplification, miniaturization, and antibodies are evaluated for the development and applications of these immunosensors. It can be concluded that despite some limitations of the immunosensing technologies, these immuosensors for pesticide monitoring are becoming more and more relevant in environmental and food analysis.
免疫传感器是一种生物传感器,它使用抗体或抗原作为特异性传感元件,并提供浓度依赖性信号。免疫传感技术在快速检测食品和环境中的农药残留方面具有巨大的应用潜力。本文概述了文献中报道的各种用于设计和制造农药检测免疫传感器的传感系统,如电化学、光学、压电和纳米力学方法。还讨论了用于形成生物识别界面的各种固定化方案。此外,对这些免疫传感器的再生、信号放大、小型化和抗体技术进行了评估,以促进其开发和应用。可以得出结论,尽管免疫传感技术存在一些局限性,但这些用于农药监测的免疫传感器在环境和食品分析中越来越重要。