Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, China.
ACS Appl Mater Interfaces. 2011 Apr;3(4):1366-73. doi: 10.1021/am200228k. Epub 2011 Apr 6.
This study demonstrates a facile and feasible strategy toward the development of advanced electrochemical immunosensors based on chemically functionalized magnetic mesoporous organic-inorganic hybrid nanomaterials, and the preparation, characterization, and measurement of relevant properties of the immunosensor for detection of carcinoembryonic antigen (CEA, as a model analyte) in clinical immunoassays. The as-prepared nanomaterials composed of a magnetic mesoporous NiCo(2)O(4) nanosheet, an interlayer of Nafion/thionine organic molecules and a nanogold layer show good adsorption properties for the attachment of horseradish peroxidase-labeled secondary anti-CEA antibody (HRP-anti-CEA). With a sandwich-type immunoassay format, the functional bionanomaterials present good analytical properties to facilitate and modulate the way it was integrated onto the electrochemical immunosensors, and allows the detection of CEA at a concentration as low as 0.5 pg/mL. Significantly, the immunosensor could be easily regenerated by only using an external magnet without the need of any dissociated reagents. Importantly, the as-synthesized magnetic mesoporous NiCo(2)O(4) nanomaterials could be further extended for detection of other biomarkers or biocompounds.
本研究展示了一种基于化学功能化磁性介孔有机-无机杂化纳米材料的先进电化学免疫传感器的简便可行的策略，以及免疫传感器的制备、表征和相关性能的测量，用于检测临床免疫分析中的癌胚抗原（CEA，作为模型分析物）。所制备的纳米材料由磁性介孔 NiCo(2)O(4)纳米片、Nafion/硫堇有机分子层和纳米金层组成，对辣根过氧化物酶标记的二级抗 CEA 抗体（HRP-anti-CEA）的附着具有良好的吸附性能。采用三明治型免疫分析形式，功能生物纳米材料具有良好的分析性能，有助于并调节其集成到电化学免疫传感器的方式，并允许以低至 0.5 pg/mL 的浓度检测 CEA。重要的是，免疫传感器可以通过仅使用外部磁铁而无需任何解离试剂轻松再生。重要的是，合成的磁性介孔 NiCo(2)O(4)纳米材料可以进一步扩展用于检测其他生物标志物或生物化合物。
