Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Lab Chip. 2011 Feb 21;11(4):723-6. doi: 10.1039/c0lc00160k. Epub 2010 Nov 22.
A novel active valving technique, whereby paraffin wax plugs in microchannels on a centrifugal microfluidic platform are actuated using focused infrared (IR) radiation is demonstrated in this report. Microchannels were simultaneously or sequentially opened using a stationary IR source by forming wax plugs with similar or differing melting points. The presented wax plugs offer key advantages over current active valving techniques, including a less involved fabrication procedure, a simpler actuation process, and the ability to multiplex experiment with active valves. In addition, a new technique for automated liquid reagent storage and release on the microfluidic disc platform, based on the formation and removal of a wax layer, is demonstrated. Overall, the techniques presented in this report offer novel methods for liquid handling, separation, and storage on the centrifugal microfluidic disc platform.
本报告展示了一种新颖的主动阀技术，该技术在离心微流控平台上的微通道中使用石蜡蜡塞，并利用聚焦的红外（IR）辐射进行驱动。通过使用具有相似或不同熔点的蜡塞，微通道可以同时或顺序打开，使用固定的 IR 源。与当前的主动阀技术相比，提出的蜡塞具有关键优势，包括更简单的制造过程、更简单的驱动过程，以及具有多路复用实验能力的主动阀。此外，还展示了一种基于蜡层形成和去除的新型自动在微流控盘平台上存储和释放液体试剂的技术。总的来说，本报告中提出的技术为离心微流控盘平台上的液体处理、分离和存储提供了新颖的方法。
