Osiri John K, Shadpour Hamed
School of Chemical Engineering & Bioengineering, Washington State University, Pullman, WA 99163, USA.
Bioanalysis. 2010 Oct;2(10):1745-54. doi: 10.4155/bio.10.134.
Profiling of protein biomarkers is powerful for the analysis of complex proteomes altered during the progression of diseases. Lab-on-a-chip technologies can potentially provide the throughput and efficiency required for point-of-care and clinical applications. While initial studies utilized 1D microchip separation techniques, researchers have recently developed novel 2D microchip separation platforms with the ability to profile thousands of proteins more effectively. Despite advancements in lab-on-a-chip technologies, very few reports have demonstrated a point-of-care microchip-based profiling of proteins. In this review, recent progress in 1D and 2D microchip profiling of protein mixtures of a biological sample with potential point-of-care applications are discussed. A selection of recent microchip immunoassay-based techniques is also highlighted.
蛋白质生物标志物分析对于分析疾病进程中发生变化的复杂蛋白质组具有强大作用。芯片实验室技术有可能提供即时医疗和临床应用所需的通量和效率。虽然最初的研究采用一维微芯片分离技术，但研究人员最近开发了新型二维微芯片分离平台，能够更有效地分析数千种蛋白质。尽管芯片实验室技术取得了进展，但很少有报告展示基于即时医疗微芯片的蛋白质分析。在本综述中，将讨论一维和二维微芯片对具有潜在即时医疗应用的生物样品蛋白质混合物进行分析的最新进展。还将重点介绍一些近期基于微芯片免疫分析的技术。
