Kim Jason S, Hur Daesung, Hwang Jeoung Ku, Chung Chanil, Chang Jun Keun
Nanoentek Inc., Ace Highend Tower, 12th floor, 235-2 Kuro-3 Dong, Kuro-Ku, Seoul, Korea.
Bioanalysis. 2010 Oct;2(10):1755-65. doi: 10.4155/bio.10.119.
Image cytometry is a method for quantitative cellular analysis using images generally captured on slides or microfabricated chips. The flowless nature of data acquisition in image cytometry allows the use of value components, such as light-emitting diode excitation sources or low-cost charge-coupled device detectors. Unlike flow cytometry, the stationary cellular samples can be exposed to lower-intensity light and utilize less sensitive detectors with higher exposure times. Images are acquired and data is processed using recognition software to identify, count and analyze cells. Current image cytometers cannot replicate the quality of the data from flow cytometers or fluorescence microscopes with full functionality and performance components. Yet, the production of inexpensive image cytometers for use in small laboratories and clinics has made a compelling argument. The addition of fluorescence detection to the new generation of image cytometers has opened the field to a broader range of applications. This article will review the technical aspects and application of image cytometers, the recent progress in the field and available commercial devices.
图像细胞术是一种用于定量细胞分析的方法，它使用通常在载玻片或微制造芯片上捕获的图像。图像细胞术中数据采集的无流动特性允许使用诸如发光二极管激发源或低成本电荷耦合器件探测器等有价值的组件。与流式细胞术不同，静止的细胞样本可以暴露于较低强度的光下，并使用曝光时间较长但灵敏度较低的探测器。采集图像并使用识别软件处理数据，以识别、计数和分析细胞。目前的图像细胞仪无法复制具有完整功能和性能组件的流式细胞仪或荧光显微镜的数据质量。然而，生产用于小型实验室和诊所的廉价图像细胞仪已经有了令人信服的理由。新一代图像细胞仪中增加荧光检测功能，为更广泛的应用领域开辟了道路。本文将综述图像细胞仪的技术方面和应用、该领域的最新进展以及现有的商业设备。
