Post-Doctoral Research Center, Affiliated Zhongshan Hospital of Xiamen University, Xiamen, Fujian Province, China.
Scand J Immunol. 2010 Dec;72(6):504-10. doi: 10.1111/j.1365-3083.2010.02466.x.
Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 μm, group A) or large (average diameter > 250 μm, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in both groups, but the pathology in group B was significantly worse. Multiple proinflammatory cytokines were significantly higher in group B than that of A at 3 h post-transplantation. Our study determined that the size of islets plays a critical role in the success of intraportal islet transplantation (IPIT) and should be taken into account in future IPIT protocols for the treatment of diabetes.
优化胰岛移植方案对于提高 1 型糖尿病治疗的成功率是必要的。在这里，我们研究了门静脉（PV）内移植的胰岛大小是否会影响实验性小鼠模型中移植后的早期植入。从小鼠（平均直径 < 250 μm，A 组）或大胰岛（平均直径 > 250 μm，B 组）中纯化的胰岛（400 胰岛当量/受者），移植到由 STZ 诱导糖尿病的同基因受体中。A 组（100%）恢复非糖尿病状态的小鼠比例高于 B 组（62.5%）。两组均观察到与胰岛栓塞相关的肝坏死灶，但 B 组的病理学更严重。移植后 3 小时，B 组的多种促炎细胞因子明显高于 A 组。我们的研究确定胰岛的大小在门静脉内胰岛移植（IPIT）的成功中起着关键作用，在未来的糖尿病治疗 IPIT 方案中应考虑这一点。
